Skip to main content

Main menu

  • Home
  • Issues
    • Issue in progress
    • Issues by date
  • Sections
    • Editorial
    • Review
    • Research
    • Commentary
    • Psychopharmacology for the Clinician
    • Letters to the Editor
  • Topic Collections
  • Instructions for Authors
    • Overview for authors
    • Submission checklist
    • Editorial policies
    • Publication fees
    • Submit a manuscript
    • Dr. Francis Wayne Quan Memorial Prize
    • Open access
  • Alerts
    • Email alerts
    • RSS
  • About
    • General information
    • Staff
    • Editorial Board
    • Contact
  • CMAJ JOURNALS
    • CMAJ
    • CMAJ Open
    • CJS
    • JAMC

User menu

Search

  • Advanced search
JPN
  • CMAJ JOURNALS
    • CMAJ
    • CMAJ Open
    • CJS
    • JAMC
JPN

Advanced Search

  • Home
  • Issues
    • Issue in progress
    • Issues by date
  • Sections
    • Editorial
    • Review
    • Research
    • Commentary
    • Psychopharmacology for the Clinician
    • Letters to the Editor
  • Topic Collections
  • Instructions for Authors
    • Overview for authors
    • Submission checklist
    • Editorial policies
    • Publication fees
    • Submit a manuscript
    • Dr. Francis Wayne Quan Memorial Prize
    • Open access
  • Alerts
    • Email alerts
    • RSS
  • About
    • General information
    • Staff
    • Editorial Board
    • Contact
  • Subscribe to our alerts
  • RSS feeds
  • Follow JPN on Twitter
Brief Report

Dimethylated lysine 9 of histone 3 is elevated in schizophrenia and exhibits a divergent response to histone deacetylase inhibitors in lymphocyte cultures

David P. Gavin, Cherise Rosen, Kayla Chase, Dennis R. Grayson, Nguwah Tun and Rajiv P. Sharma
J Psychiatry Neurosci May 01, 2009 34 (3) 232-237;
David P. Gavin
Gavin, Rosen, Chase, Grayson, Tun, Sharma — The Psychiatric Institute, University of Illinois at Chicago; Gavin, Rosen, Grayson, Sharma — Department of Psychiatry, University of Illinois at Chicago College of Medicine, Chicago, Ill.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Cherise Rosen
Gavin, Rosen, Chase, Grayson, Tun, Sharma — The Psychiatric Institute, University of Illinois at Chicago; Gavin, Rosen, Grayson, Sharma — Department of Psychiatry, University of Illinois at Chicago College of Medicine, Chicago, Ill.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Kayla Chase
Gavin, Rosen, Chase, Grayson, Tun, Sharma — The Psychiatric Institute, University of Illinois at Chicago; Gavin, Rosen, Grayson, Sharma — Department of Psychiatry, University of Illinois at Chicago College of Medicine, Chicago, Ill.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Dennis R. Grayson
Gavin, Rosen, Chase, Grayson, Tun, Sharma — The Psychiatric Institute, University of Illinois at Chicago; Gavin, Rosen, Grayson, Sharma — Department of Psychiatry, University of Illinois at Chicago College of Medicine, Chicago, Ill.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Nguwah Tun
Gavin, Rosen, Chase, Grayson, Tun, Sharma — The Psychiatric Institute, University of Illinois at Chicago; Gavin, Rosen, Grayson, Sharma — Department of Psychiatry, University of Illinois at Chicago College of Medicine, Chicago, Ill.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
Rajiv P. Sharma
Gavin, Rosen, Chase, Grayson, Tun, Sharma — The Psychiatric Institute, University of Illinois at Chicago; Gavin, Rosen, Grayson, Sharma — Department of Psychiatry, University of Illinois at Chicago College of Medicine, Chicago, Ill.
  • Find this author on Google Scholar
  • Find this author on PubMed
  • Search for this author on this site
  • Article
  • Figures & Tables
  • Responses
  • Metrics
  • PDF
Loading

Article Figures & Tables

Figures

  • Tables
  • Fig. 1
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 1

    To establish assay sensitivity of the Western blot analysis, we measured dimethylated lysine 9 of histone 3 (H3K9me2) and total histone 1 (H1) in serial dilutions (0.50, 0.25, 0.125 and 0.0625 μg/μL) of initial input protein. (A) Graphical results of H3K9me2 values across the protein concentration range plotted against H1. Theoretically, the r2 should be 1; r2 = 0.9957. (B) Representative Western blot of H3K9me2 and H1 across 2-fold dilutions.

  • Fig. 2
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 2

    (A) Elevated dimethylated lysine 9 of histone 3 (H3K9me2) levels in patients with schizophrenia compared with healthy controls. We measured H3K9me2 using equal amounts of protein appropriately transferred to a nitrocellulose membrane and detected it with an Anti-dimethyl-Histone H3 (Lys 9) (H3K9me2) rabbit polyclonal antibody (Millipore #07-521). We then normalized it to total histone 1 (H1) using Anti-Histone H1, clone AE-4 (total H1) mouse monoclonal antibody (Millipore #05-457). Error bars represent standard errors of the mean. (B) Correlation between onset of psychiatric symptoms and H3K9me2. Earlier age of onset is associated with higher levels of H3K9me2 in patients with schizophrenia.

  • Fig. 3
    • Download figure
    • Open in new tab
    • Download powerpoint
    Fig. 3

    (A) After 24 hours of incubation with the histone deacetylase inhibitor, trichostatin A (TSA) H3K9me2 levels respond differently in lymphocyte cultures from patients with schizophrenia compared with cultures from healthy controls. Error bars represent standard errors of the mean. (B) Representative Western blot showing a different TSA-induced (relative to dimethyl sulfoxide [control, CT]) response in H3K9me2 in a sample with schizophrenia compared with healthy controls.

Tables

  • Figures
    • View popup
    Table 1

    Diagnostic, medication status and demographic characteristics of patients with schizophrenia and healthy controls

    CharacteristicGroup, mean (SD)*
    Schizophreni a, n = 25Control, n = 19
    Sex, female:male6:196:13
    Age, yr33.3 (11.2)32.9 (9.7)
    Age at onset of disease, yr17.6 (8.0)NA
    Duration, yr14.9 (11.4)NA
    Antipsychotic medication, %NA
     Typical12
     Atypical52
     Typical + atypical4
     None32
    • NA = not applicable; SD = standard deviation.

    • ↵* Unless otherwise indicated.

PreviousNext
Back to top

In this issue

Journal of Psychiatry and Neuroscience: 34 (3)
J Psychiatry Neurosci
Vol. 34, Issue 3
1 May 2009
  • Table of Contents
  • Table of Contents (PDF)
  • Index by author

Article tools

Respond to this article
Print
Download PDF
Article Alerts
To sign up for email alerts or to access your current email alerts, enter your email address below:
Email Article

Thank you for your interest in spreading the word on JPN.

NOTE: We only request your email address so that the person you are recommending the page to knows that you wanted them to see it, and that it is not junk mail. We do not capture any email address.

Enter multiple addresses on separate lines or separate them with commas.
Dimethylated lysine 9 of histone 3 is elevated in schizophrenia and exhibits a divergent response to histone deacetylase inhibitors in lymphocyte cultures
(Your Name) has sent you a message from JPN
(Your Name) thought you would like to see the JPN web site.
CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Citation Tools
Dimethylated lysine 9 of histone 3 is elevated in schizophrenia and exhibits a divergent response to histone deacetylase inhibitors in lymphocyte cultures
David P. Gavin, Cherise Rosen, Kayla Chase, Dennis R. Grayson, Nguwah Tun, Rajiv P. Sharma
J Psychiatry Neurosci May 2009, 34 (3) 232-237;

Citation Manager Formats

  • BibTeX
  • Bookends
  • EasyBib
  • EndNote (tagged)
  • EndNote 8 (xml)
  • Medlars
  • Mendeley
  • Papers
  • RefWorks Tagged
  • Ref Manager
  • RIS
  • Zotero
‍ Request Permissions
Share
Dimethylated lysine 9 of histone 3 is elevated in schizophrenia and exhibits a divergent response to histone deacetylase inhibitors in lymphocyte cultures
David P. Gavin, Cherise Rosen, Kayla Chase, Dennis R. Grayson, Nguwah Tun, Rajiv P. Sharma
J Psychiatry Neurosci May 2009, 34 (3) 232-237;
Digg logo Reddit logo Twitter logo Facebook logo Google logo Mendeley logo
  • Tweet Widget
  • Facebook Like

Related Articles

  • No related articles found.
  • PubMed
  • Google Scholar

Cited By...

  • No citing articles found.
  • Google Scholar

Similar Articles

Content

  • Current issue
  • Past issues
  • Collections
  • Alerts
  • RSS

Authors & Reviewers

  • Overview for Authors
  • Submit a manuscript
  • Manuscript Submission Checklist

About

  • General Information
  • Staff
  • Editorial Board
  • Contact Us
  • Advertising
  • Reprints
  • Copyright and Permissions
  • Accessibility
  • CMA Civility Standards
CMAJ Group

Copyright 2022, CMA Impact Inc. or its licensors. All rights reserved. ISSN 1180-4882.

All editorial matter in JPN represents the opinions of the authors and not necessarily those of the Canadian Medical Association or its subsidiaries.
To receive any of these resources in an accessible format, please contact us at CMAJ Group, 500-1410 Blair Towers Place, Ottawa ON, K1J 9B9; p: 1-888-855-2555; e: [email protected].
View CMA's Accessibility policy.

Powered by HighWire