TY - JOUR T1 - <em>S100A10</em> identified in a genome-wide gene × cannabis dependence interaction analysis of risky sexual behaviours JF - Journal of Psychiatry and Neuroscience JO - J Psychiatry Neurosci SP - 252 LP - 261 DO - 10.1503/jpn.160189 VL - 42 IS - 4 AU - Renato Polimanti AU - Shashwath A. Meda AU - Godfrey D. Pearlson AU - Hongyu Zhao AU - Richard Sherva AU - Lindsay A. Farrer AU - Henry R. Kranzler AU - Joel Gelernter Y1 - 2017/07/01 UR - http://jpn.ca/content/42/4/252.abstract N2 - Background: We conducted a genome-wide gene × environment interaction analysis to identify genetic variants that interact with cannabis dependence (CaD) in influencing risky sexual behaviours (RSB).Methods: Our sample included cannabis-exposed and sexually experienced African-American and European-American participants. A DSM-IV CaD diagnosis and RSB were evaluated using the Semi-Structured Assessment for Drug Dependence and Alcoholism. We analyzed RSBs as a score that takes into account experiences of unprotected sex and multiple sexual partners.Results: A total of 3350 people participated in our study; 43% had a CaD diagnosis, 56% were African-American and 33% were women. We identified a genome-wide significant locus in African-American participants (S100A10 rs72993629, p = 2.73 × 10−8) and a potential transpopulation signal in women (CLTC rs12944716, p = 5.27 × 10−8). A resting-state fMRI follow-up analysis of S100A10 rs72993629 conducted in an independent cohort showed 2 significant associations: reduced power of the left paracentral lobule in amplitude of low frequency fluctuations (ALFF) analysis (p = 7.8 × 10−3) and reduced power of the right pallidum in fractional ALFF analysis (p = 4.6 × 10−3). The activity of these brain regions is known to be involved in sexual functions and behaviours. The S100A10 result functionally recapitulated our S100B finding observed in our previous genome-wide association study of CaD. The probability of identifying 2 S100 genes in 2 independent genome-wide investigations by chance is approximately 1 in 1.1 million.Limitations: We were not able to identify any African-American cohort with appropriate sample size, and phenotypic assessment is available to replicate our findings.Conclusion: The S100A10 and S100B genes, which are located on different chromosomes, encode specialized calcium-binding proteins. These data support a role for calcium homeostasis in individuals with CaD and its induced behaviours. ER -