Average expression of interesting splicing factor genes in OVX rats under different treatment conditions
| Early ET; mean ± SEM (n = 6) | Late ET; mean ± SEM (n = 5) | |||||
|---|---|---|---|---|---|---|
| Gene | OVX + V | OVX + E2 | OVX + V | OVX + E2 | OVX + DPN | OVX + PPT |
| SFRS7 | 3.09 ± 0.65# | 1.37 ± 0.22* | 0.58 ± 0.06# | 0.58 ± 0.16 | 0.99 ± 0.05* | 0.82 ± 0.03#* |
| SFRS16 | 1.61 ± 0.20# | 0.64 ± 0.06#* | 1.17 ± 0.07 | 4.94 ± 0.66#* | 1.52 ± 0.39 | 3.82 ± 1.55 |
| ZRSR2 | 0.46 ± 0.05# | 2.05 ± 0.5* | 0.31 ± 0.03# | 2.51 ± 0.36#* | 1.69 ± 0.08* | 0.38 ± 0.14 |
| CTNNB1 | 0.89 ± 0.03# | 1.32 ± 0.07#* | 0.76 ± 0.03# | 1.26 ± 0.24 | 1.57 ± 0.26* | 1.05 ± 0.11 |
ANOVA = analysis of variance; DPN = diarylpropionitrile; E2 = estradiol; ET = estrogen therapy; OVX = ovariectomy; PPT = propylpyrazoletriol; qPCR = quantitative polymerase chain reaction; SEM = standard error of the mean; V = vehicle.
↵# p < 0.05 v. sham + V;
↵* p < 0.05 v. OVX + V.
Fold changes of the qPCR results were analyzed with 1-way ANOVA and a subsequent Bonferroni post hoc test.