Ucn1 mRNA is a putative miR-326 target
| Target site; species | Position | Target sequence | p value* |
|---|---|---|---|
| miR-326/Ucn1 | |||
| Human | 45–51 of Ucn1 3′-UTR hsa-miR-326 | 5′-ACCCCUUUCCCCCACCCCAGAGU 3′-GACCUCCUUCCCGGGUCUCC | 0.008 |
| Rat | 104–110 of Ucn1 3′-UTR rno-miR-326 | 5′-CGCGUUGCAGCGCCGCCCAGAGU 3′-UGACCUCCUUCCCGGGUCUCC | 0.002 |
| Mouse | 104–110 of Ucn1 3′-UTR mmu-miR-326 | 5′-CGCGGUGCAGCGCCGCCCAGAGU 3′-UGACCUCCUUCCCGGGUCUCC | 0.003 |
| miR-326/CART | |||
| Human | 45–51 of CART 3′-UTR hsa-miR-326 | 5′-CAUCCCUCUACUUUCCCCAGAGG 3′-GACCUCCUUCCCGGGUCUCC | 0.007 |
| Rat | 104–110 of CART 3′-UTR rno-miR-326 | 5′-AAAGUUUGCGUUCCCCCCAGAGA 3′-UGACCUCCUUCCCGGGUCUCC | 0.026 |
| Mouse† | 104–110 of CART 3′-UTR mmu-miR-326 | 5′-AAAGUUUGCGUUCCCCUCAGAGA 3′-UGACCUCCUUCCCGGGUCUCC | N/A |
| miR-325/CART | |||
| Human‡ | 83–89 of CART 3′-UTR hsa-miR-325 | 5′-GAGUUUGGCUUAAGCAACAGAUAAAG 3′-CCUAGUAGGUGUCCAGUAAGUGU | N/A |
| Rat | 104–110 of CART 3′-UTR rno-miR-326 | 5′-GAGCCGCUUUAACAACAAUAAAG 3′-AACUAUCCUCCACGAGUUAUUU | 0.010 |
| Mouse | 104–110 of CART 3′-UTR mmu-miR-326 | 5′-GAGCCUGGCUUUAGCAACAAUAAA 3′-AACUAUCCUCCACGAGUUAUUU | 0.36 |
CART = cocaine- and amphetamine-regulated transcript; Ucn1 = urocortin 1; UTR = untranslated region.
↵* Probability distribution of random matches of a subsequence (miR 5′ end sequence) in the given sequence (promoter or mRNA sequence) calculated using Poisson distribution, where a low probability implies a significant hit.
↵† Note the absence of miR-326 binding site in mouse CART 3′-UTR.
↵‡ Note the absence of miR-325 binding possibility in human CART 3′-UTR.