TaqMan primers/probes used for real-time polymerase chain reaction analysis
| Primer/probe | TaqMan accession | Probe location (exon boundary) | Assay function |
|---|---|---|---|
| ACTB | Hs99999903_m1 | 1–1 | Housekeeping gene |
| GAPDH | Hs99999905_m1 | 3–3 | Housekeeping gene |
| TNF-α | Hs99999043_m1 | 1–2 | Target gene |
| LTA (TNF-β) | Hs99999086_m1 | 3–4 | Target gene |
| LTB | Hs00242737_m1 | 1–2 | Target gene |
| IL-1β | Hs01555410_m1 | 3–4 | Target gene |
| IL-6 | Hs00985639_m1 | 2–3 | Target gene |
| IL-1RN (IL-1RA) | Hs00893626_m1 | 4–5 | Target gene |
| IL-8 | Hs00174103_m1 | 1–2 | Target gene |
| IL-10 | Hs00961622_m1 | 4–5 | Target gene |
| IL-13 | Hs00174379_m1 | 1–2 | Target gene |
ACTB = β-actin; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; IL = interleukin; LTA = lymphotoxin A; LTB = lymphotoxin B; TNF = tumour necrosis factor.