Primers/probes used for real-time PCR analysis
| Primer/probe | TaqMan accession | Probe location (exon boundary) | Assay function |
|---|---|---|---|
| ACTB | Hs99999903_m1 | 1–1 | Housekeeping gene |
| GAPDH | Hs99999905_m1 | 3–3 | Housekeeping gene |
| NLRP1 | Hs00248187_m1 | 6–7 | Target gene |
| NLRP3 | Hs00918082_m1 | 1–2 | Target gene |
| NLRP6 | Hs00373246_m1 | 5–6 | Target gene |
| CASP1 | Hs00354836_m1 | 8–9 | Target gene |
| CASP3 | Hs00234387_m1 | 4–5 | Target gene |
| PYCARD/ASC | Hs00203118_m1 | 2–3 | Target gene |
| IL18 | Hs01038788_m1 | 3–4 | Target gene |
PCR = polymerase chain reaction.