Primers used to amplify target loci for RT-PCR
| Gene amplified | Nucleotide sequence of primer | Primer type | Product length (bp) | NCBI reference sequence |
|---|---|---|---|---|
| Hs TRPA1 | TCCTCTCCATCTGGCAGCAAAG | Forward | 115 | NM_007332.3 |
| GGACGCATGATGCAAAGCTGTC | Reverse | |||
| Hs POLR2A | GAGAGCGTTGAGTTCCAGAACC | Forward | 152 | NM_000937.5 |
| TGGATGTGTGCGTTGCTCAGCA | Reverse | |||
| Hs UCN1 | CTTCTCTGTCCATTGACCTCACC | Forward | 123 | NM_003353.4 |
| ATCACTTGCCCACCGAGTCGAA | Reverse | |||
| Mm Trpa1 | ATCCAAATAGACCCAGGCACG | Forward | 101 | NM_177781.5 |
| CAAGCATGTGTCAATGTTTGGTACT | Reverse | |||
| Mm Actb | CTGTATGCCTCTGGTCGTAC | Forward | 214 | NM_007393.5 |
| TGATGTCACGCACGATTTCC | Reverse | |||
| Mm Gapdh | TTCACCACCATGGAGAAGGC | Forward | 237 | NM_008084.3 |
| GGCATGGACTGTGGTCATGA | Reverse |
Hs = Homo sapiens; Mm = Mus musculus; NCBI = National Center for Biotechnology Information; RT-PCR = reverse transcription polymerase chain reaction.