Elsevier

Brain Research

Volume 662, Issues 1–2, 31 October 1994, Pages 245-250
Brain Research

Short communication
Neuron-specific human glutamate transporter: molecular cloning, characterization and expression in human brain,☆☆

https://doi.org/10.1016/0006-8993(94)90819-2Get rights and content

Abstract

A cDNA encoding a neuron-specific glutamate/aspartate transporter was isolated from human brain cDNA libraries and characterized. The new cDNA, designated human glutamate transporter III, is structurally distinct from two previously described brain specific glutamate transporters. This human cDNA is 90% and 95% homologous at nucleotide and amino acid level, respectively, with a previously reported rabbit glutamate/aspartate transporter. Northern blot analysis of human tissues revealed that the mRNA of this transporter is expressed in brain, liver, muscle, ovary, testis and in retinoblastoma cell lines. In situ hybridization in human brain sections showed that the mRNA is densely expressed in substantia nigra, red nucleus, hippocampus, and in cerebral cortical layers. Southern blot analysis revealed that the gene encoding this mRNA exists as a single copy in the human genome.

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The sequence data reported in this paper have been submitted to the GenBank data libraries under the accession no. U08989.

☆☆

This work was supported by NIH Grants NS-16871 (A.P.), AG06647 (J.H.M.); by a grant from the International Federation of Parkinson's Disease Societies (P.S.). G.W.H. is supported by Aaron Diamond Foundation and by Alexandrine and Alexander Sinsheimer Fund. T.M. was an exchange student under the auspices of Biomedical Exchange Program (BMEP) between the USA and Germany. We thank Nancy Archin for expert technical assistance, and Betsy Chalfin for editorial assistance.

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