Elsevier

Neuroscience Letters

Volume 133, Issue 2, 9 December 1991, Pages 183-186
Neuroscience Letters

Neurotensin and growth hormone-releasing factor-containing neurons projecting to the median eminence of the rat: A combined retrograde tracing and immunohistochemical study

https://doi.org/10.1016/0304-3940(91)90565-BGet rights and content

Abstract

The origins of the neurotensin (NT)-containing nerve terminals in the median eminence were determined in rats by a combination of retrograde labeling with True blue and immunofluorescence staining. Moreover, the distribution of NT and growth hormone-releasing factor (GRF) was analyzed in the arcuate nucleus (ARC) with an elution-restaining procedure. The retrogradely labeled NT neurons were found mainly in the ARC. Only a few labeled NT neurons were seen in the periventricular and the paraventricular nuclei. The elution restaining procedure demonstrated that an average of 43% of the labeled NT cells in the ventral part of the ARC restained for GRF. These findings support the hypothesis of co-expression of NT with GRF from the ARC.

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