Elsevier

Neuroscience Letters

Volume 234, Issue 1, 26 September 1997, Pages 71-73
Neuroscience Letters

Beta-amyloid induced increase in choline flux across PC12 cell membranes

https://doi.org/10.1016/S0304-3940(97)00671-XGet rights and content

Abstract

Beta-amyloid peptide is the main constituent of senile plaques and is implicated in the pathogenesis of Alzheimer's disease. It has been shown to be both neurotoxic and neurotrophic in vivo, and its effects have been suggested to be mediated in part by alterations in membrane transport. In the present study, we investigated the effect of beta-amyloid (1–40) on choline transport in cultured PC12 cells. We found that exposure to 46 or 92 μM beta-amyloid (1–40) increased [14C]choline flux in PC12 cells in a concentration-dependent manner, whereas exposure to reverse sequence beta-amyloid (40–1) had no effect. If there is a similar effect in vivo, the increased beta-amyloid dependent permeability to choline could lead to depletion of cellular choline stores and could contribute to the selective vulnerability of cholinergic neurons in Alzheimer's disease.

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Acknowledgements

This work was supported by the NIA Intramural Program. The authors would like to thank Dr. Gerald Ehrenstein for inspiration and insightful discussions and Dr. Kishena Wadhwani for his help at the beginning of the project.

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1

Present address: Department of Dynamic Pathology, Research Institute of Neurological Diseases and Geriatrics, Kyoto Prefectural University of Medicine, 465 Kajii, Hirokoji, Kawaramachi, Kyoto, 602 Japan.

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