Estrogen binding and estrogen receptor characterization (ERα and ERβ) in the cholinergic neurons of the rat basal forebrain
Section snippets
Materials
125I-estrogen (17α-iodovinyl-11β-methoxyestradiol) was obtained from the custom iodination (NEN; Boston, MA, U.S.A.) of E-17-tributylstannyvinyl-11-methoxy-estradiol (RAXL Enterprises; Newton, MA, U.S.A.) as described previously.17
Animals
In the 125I-estrogen binding studies described below, ovariectomized rats were used because endogenous estrogens would compete with the radiolabeled ligand and potentially mask estrogen binding sites. Rats used for the double-label in situ
In vivo binding/immunocytochemistry studies
The present study used 17α-iodovinyl-11β-methoxyestradiol (125I-estrogen), a new radiolabeled estrogen, for in vivo binding studies because this compound has recently been shown to: (i) have a similar binding affinity for both ERα and ERβ in vitro and in vivo; (ii) be selective for ERs; (iii) be slowly metabolized and; (iv) exhibit a good signal–noise profile in vivo.17., 33. Through the use of a double-label in vivo binding/immunocytochemistry technique, the co-localization of estrogen
Discussion
The results of the present co-localization studies have demonstrated that ChAT neurons in the rat basal forebrain nuclei contain a biologically active ER and shown that ERα is the predominant ER in these ChAT neurons. Through the use of a combined in vivo autoradiography/immunocytochemistry technique, we found that 22–41% of the ChAT neurons in the medial septum and diagonal band of Broca had a nuclear uptake and retention of radiolabeled estrogen (125I-estrogen), while only 4% of the ChAT
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2023, PsychoneuroendocrinologyGenistein inhibits amyloid peptide 25-35-induced neuronal death by modulating estrogen receptors, choline acetyltransferase and glutamate receptors
2020, Archives of Biochemistry and BiophysicsCitation Excerpt :In healthy older women, estrogen pretreatment attenuated anticholinergic drug-induced cognitive impairments, demonstrating the interaction of estrogen and the cholinergic system and the effects on cognitive performance in humans [59]. Biologically active ERα and ERβ are present in the basal forebrain cholinergic neurons of the adult rat brain, and this finding is consistent with the possibility that estrogen directly modulates the activity of cholinergic neurons in rats [60]. In addition, the majority of cholinergic neurons in the forebrain exhibit GPR30-like immunoreactivity, and activation of GPR30 enhances cholinergic function in the hippocampus [16].
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