Original articleSerotonin 5-HT1A, 5-HT1B, and 5-HT2A receptor mRNA expression in subjects with major depression, bipolar disorder, and schizophrenia
Section snippets
Subjects
Sixty subjects from the Stanley Foundation Neuropathology Consortium were analyzed in this project. Postmortem brains comprised four groups of 15 subjects each, with diagnoses of schizophrenia, bipolar disorder, major depression disorder without psychotic features, and a normal comparison group. For a detailed description of subject selection, diagnostic methods, and tissue handling, see Torrey et al (2000).
Samples were matched for age (overall mean ± SEM: 45.4 ± 1.7), gender, ethnicity, side
5-HT1A mRNA
Densitometric analysis of the distribution of 5-HT1A mRNA in the human DLPFC shows a pattern of four bands (Figure 1A). In the control group, labeling was more intense in B1, lower in B2 (approximately half of B1) and B4 (approximately a quarter of B1), and the lowest was B3 (approximately a 10th of B1, Figure 2A).
In the HC, 5-HT1A mRNA was present in all the regions analyzed (CA1, CA2, CA4, and DG), with the highest levels in CA1, followed by CA2 and DG (with approximately half of CA1's
Distribution of 5-HT1A, 5-HT1B, and 5-HT2A in the dorsolateral prefrontal cortex and hippocampus
The expression pattern of each mRNA followed a different pattern of hybridization within the DLPFC and HC. In the DLPFC the “densitometric” patterns are referred to as bands to differentiate these patterns from the already characterized and well-described anatomic cortical layers. The bands might not correspond exactly with the number, extension, and distribution of the classical layers. As described subsequently, the results are consistent with previous descriptions, but we note that the bands
Acknowledgements
We acknowledge the support of Drs. James H Meador-Woodruff, Charles Neal Jr., Marco Cecchi, Mohamed Kabbaj, Mónica Beneyto Santonja, Inés Morano, Lucio Díaz-Flores Feo, Tomás González Hernández, and Elena Vigo Moreno. Postmortem brains were donated by the Stanley Medical Research Institute's Brain Collection, courtesy of Drs. Michael Knable, E. Fuller Torrey, Maree J. Webster, Serge Weis, and Robert H. Yolken.
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