Original articleAcute Occupancy of Brain Serotonin Transporter by Sertraline as Measured by [11C]DASB and Positron Emission Tomography
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Subjects
Inclusion criteria were assessed through history, Structured Clinical Interview for DSM IV (First 1997), review of systems, physical examination, routine blood tests, pregnancy test, urine toxicology and EKG. Inclusion criteria included: 1) age 18 to 65 years; 2) no Axis I DSM-IV diagnoses; 3) absence of any psychotropic medications for at least 2 weeks; 4) absence of lifetime history of alcohol or substance abuse or dependence; 5) absence of life-time exposure to
Determination of a Reference Region
Due to low but measurable 5-HTT binding in the cerebellum (Kent et al 2002, Kretzschmar et al 2003, Szabo et al 2002), we examined a human cerebellum using [3H]cyanoimipramine autoradiography to suggest the best location to sample in order to minimize specific binding (Figure 1). Specific 5-HTT binding is much higher in the cerebellar vermis (CV, 8.4 fmol/mg) compared with cerebellar gray matter (CG, 1.25 fmol/mg), and cerebellar white matter (CW, .23 fmol/mg).
In in vivo brain imaging studies,
Discussion
Previous in vivo measures of 5-HTT occupancy have reported occupancy of 5-HTT after chronic SSRI intake (Meyer et al 2001, Meyer et al 2004). As we have several methodological differences with these publications, in order to compare our results we analyzed our data as close to possible as was done previously. We apply the graphical approach using CT instead of CG from each scan as the reference region in calculating BP2. With these modifications, we found a lesser degree of occupancy. For
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