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World J Psychiatr. Dec 22, 2012; 2(6): 124-133
Published online Dec 22, 2012. doi: 10.5498/wjp.v2.i6.124
Platelets: A possible glance into brain biological processes in schizophrenia
Eyal Asor, Dorit Ben-Shachar, Laboratory of Psychobiology, Department of Psychiatry, Rambam Medical Center and B. Rappaport Faculty of Medicine, Rappaport Family Institute for Research in the Medical Sciences, Technion, PO Box 9649, Haifa 31096, Israel
Author contributions: Asor E and Ben-Shachar D both wrote this review.
Correspondence to: Dr. Dorit Ben-Shachar, Laboratory of Psychobiology, Department of Psychiatry, Rambam Health Care Campus and B. Rappaport Faculty of Medicine, Technion ITT, PO Box 9649, Haifa 31096, Israel. shachar@tx.technion.ac.il
Telephone: +972-4-8295224 Fax: +972-4-8295220
Received: November 11, 2011
Revised: July 2, 2012
Accepted: July 23, 2012
Published online: December 22, 2012

Abstract

Schizophrenia is a severe mental disorder, characterized by behavioral, emotional and cognitive disturbances, which commonly follows a chronic course. Diagnostic accuracy, management plans, treatment evaluation and prognosis are dependent on relatively subjective assessments. Despite extensive research and improvement in imaging technology, as well as modern genetic and molecular methodologies, the biological basis of this disease is still unclear. Therefore, there is a need for objective and valid biological markers. Platelets have often been used as a model in neurobiological research. The accessibility of platelets and their similarities with neurons turns them into an attractive candidate to search for biological markers for diagnosis and for unraveling pathophysiological processes relevant to the etiology of brain disorders, including schizophrenia. The present review addresses the main changes in platelet physiology observed in schizophrenia and its response to antipsychotic medication. We summarize numerous studies demonstrating impaired metabolism, uptake and receptor kinetics of schizophrenia-relevant neurotransmitters, abnormalities in membrane derived phospholipids and polyunsaturated fatty acids, as well as dysfunctions in the mitochondria. These changes fit with the various hypotheses raised for the etiology of schizophrenia, including the dopamine-glutamate hypothesis, the autoimmune hypothesis, the polyunsaturated fatty acid hypothesis and the impaired energy metabolism hypothesis. Despite extensive research in platelets, no conclusive reliable biomarker has been identified yet. This review suggests that the clinical heterogeneity and the biological complexity of schizophrenia lead to the inevitable conclusion that biomarkers will be identified only for subgroups characterized according to the different diagnostic criteria. Moreover, any biomarker would have to be an array of interrelated factors or even a set of several such arrays.

Key Words: Platelets, Schizophrenia, Biomarkers



INTRODUCTION

Schizophrenia is a devastating psychiatric disorder with diverse clinical manifestations. Life-time prevalence of this disorder is approximately 1% and it commonly follows a chronic course with an onset at late adolescence. Schizophrenia is characterized by cognitive, emotional and behavioral abnormalities[1-3]. These psychological and behavioral characteristics are associated with a variety of impairments in occupational and social functioning. The characteristic symptoms of the disease are often conceptualized as falling into two broad categories, positive symptoms and negative (or deficit) symptoms, with a third category, disorganized, recently added. The positive symptoms include delusions and hallucinations. Negative symptoms include restricted range and intensity of emotional expression (affective flattening), reduced thought and speech productivity (alogia), anhedonia and decreased initiation of goal-directed behavior (avolition)[4]. Disorganized symptoms include disorganized speech[5], disorganized behavior and poor attention. No single symptom is pathognomonic of schizophrenia. The disorder is noted for its great heterogeneity across individuals and for its variability within individuals over time, with alternations between psychotic episodes and residual states[6-8]. Diagnostic accuracy, prognostication, management plans and treatment evaluation are dependent on relatively subjective assessments. Therefore, there is a need for objective and valid biological markers. This need was raised at least six decades ago when the first antipsychotic drug was introduced, what we call the biological era in psychiatry. Despite extensive research and improvement in imaging technology, as well as modern genetic and molecular methodologies, the biological basis of this disease is still unclear. The heterogeneity of patient population, the diverse characteristics of schizophrenia itself, as well as confusion in diagnostic criteria, adds obstacles in the endeavor to reach this goal. In addition, the long-term therapy of schizophrenia frequently causes numerous effects which further complicate laboratory research.

The last decades have seen rapid progress in the development of noninvasive technologies to study human brain structure and function, yet there remain substantial limitations in our ability to investigate the human brain. Therefore, many experimental attempts have been made to generate reliable blood-derived candidate biomarkers based on the current models of disease pathogenesis. Platelets have often been used as a model in neurobiological research. Platelets and neurons do not share a common embryological origin as the platelets are formed from megakaryocytes that originate from the mesoderm and the neurons are formed from the ectoderm. Regardless of their embryonic origin, platelets and neurons demonstrate similar pathologies in many neuropsychiatric diseases, including Alzheimer’s and Parkinson’s diseases, schizophrenia and major depression. Platelets, like brain cells, are part of the amine precursor uptake and decarboxylation system described by Pearse[9]. Other similarities that rationalize the use of platelets as a model for neuronal biochemistry include: active transport system for serotonin (5-HT)[10], binding sites for many drugs and neurotransmitters, dense bodies which store 5-HT, and mitochondria expressing monoamine oxidase-B (MAO-B). In addition, drugs that affect the metabolism and release of CNS amines, including dopamine, 5-HT and norepinephrine, also release amines from platelets[11]. The interest in platelets as a tool for schizophrenia research arose a few years after the isolation of serotonin from the serum in 1948[12] and the observation of Gaddum et al[13] that the peripheral effects of serotonin could be antagonized by the known psychotomimetic drug Lysergic acid diethylamide (LSD). By the end of the 50s, the first studies of platelet serotonin concentration in schizophrenic patients had been published, demonstrating high levels of platelet 5HT in psychosis[14,15]. The second important step was the report by Murphy et al[16] in 1972 on lower platelet MAO activity in patients with schizophrenia compared to healthy subjects, which opened an era of MAO activity research in psychiatry. Numerous studies have shown that platelets from schizophrenic patients behave differently than those isolated from healthy controls in dopamine uptake, 5-HT content arachidonic acid metabolism, inositol phosphate levels and disturbance of calcium homeostasis[17-19]. The accessibility of platelets and their similarities with neurons turn them into an attractive candidate to search for biological markers for diagnosis and for unraveling pathophysiological processes relevant to the etiology of schizophrenia. Numerous studies have used platelets in attempt to identify a biomarker for various neuropsychiatric disorders, including Alzheimer’s and Parkinson’s disease, as well as major depression. This article will focus on the main findings in platelets in schizophrenia. These studies in platelets have been undertaken in an attempt to develop a biomarker for this heterogeneous disorder. While the data in platelet research did not pinpoint a clear single biomarker, there is mounting evidence of multiple impaired factors. Combining these factors into a single array may produce a profile that converge multiple biological abnormalities that will contribute to the diagnosis and to novel treatment strategies in schizophrenia.

SCHIZOPHRENIA RELEVANT NEUROTRANSMITTER SYSTEMS
MAO activity

Monoamine oxidase (MAO) is a mitochondrial bound enzyme, which catalyzes the oxidative deamination of a variety of monoamines, including dopamine, 5-HT and noradrenaline. MAO exists in two catalytically active forms, MAO-A and MAO-B, encoded by separate genes located on chromosome X[20]. In brain, the ratio of the two enzymes is in favor of MAO-B, while platelets contain only the MAO-B form[21].

Two decades after the initial report by Murphy and Wyatt in 1972 on lower platelet MAO activity in patients with schizophrenia compared to healthy subjects[16], numerous studies were performed to replicate their findings or refine them to specific sub-groups of schizophrenia patients.

MAO activity shows gender differences and is lower in men than in women[22]. During the life span, it tends to increase with age[23] and in women, MAO activity is decreased during the premenstrual phase[24], in pregnancy and post-partum[25]. In the general population, reduced platelet MAO activity was found to correlate with neurotic personality, psychiatric morbidity[26] and suicidal behavior[27]. In patients with psychiatric disorders, MAO activity was found to be reduced in bipolar depression compared to unipolar depression[28], higher in anxiety states[29] and Alzheimer’s disease[30], and lower in alcoholism[31] and schizophrenia (Table 1).

Table 1 Variations in monoamine oxidase activity in health and disease.
High platelets MAO activityLow platelets MAO activity
WomenMen
ElderlyPremenstrual phase in woman
Anxiety statesBipolar disorder
Alzheimer diseaseAlcoholism
Schizophrenia

In schizophrenia, genetic studies in monozygotic and dizygotic twins indicated a genetic control[32]. For example, platelet MAO activity, determined in 102 patients with chronic schizophrenia, 223 first-degree relatives and 88 normal control subjects, was shown to be a heritable and stable trait and was significantly lower in patients than in normal control subjects[33]. Using the transmission probability model, the familial transmission of MAO activity was consistent with either recessive or additive inheritance, but not with dominant inheritance[34]. Between schizophrenic patients, significantly lower MAO activity was found in chronic patients with a family history of schizophrenia compared to schizophrenics with no affected relatives and to normal controls[35]. Within families, MAO activity distinguished ill from well relatives. However, the considerable overlap in enzyme activity between affected and unaffected individuals limited the usefulness of low MAO activity as a major risk factor or a biomarker in schizophrenia[33]. In contrast, other studies did not find a significant difference between drug-free schizophrenic patients with or without a family history of the illness and healthy normal controls[36]. In addition, a study in children with schizophrenia found no significant correlation in MAO activity between patients and their parents[37].

In order to establish a clinical relevance for platelet MAO activity, the effect of antipsychotic drugs was also studied. In a meta-analysis study of fifty studies which were published through the 70s and 80s on platelet MAO in schizophrenia, it was shown that, in most studies, antipsychotic medicated schizophrenia patients had lower platelet MAO activity than healthy controls[38]. Indeed, administration of antipsychotic drugs was shown to lower MAO activity. For example, in platelets derived from patients after 14 and 21 d of haloperidol treatment, MAO activity was significantly reduced, yet did not correlate with the response to treatment. In vitro studies confirmed the ability of haloperidol to decrease MAO activity[39,40]. In drug-free schizophrenic patients, only a minority of studies found a decrease in platelet MAO in patients compared to healthy controls, while most studies did not observe any difference between the groups[38].

The inconclusive data regarding MAO activity reduction in schizophrenia and the heterogeneity of the disease led researchers to look at subgroups and specific symptoms of the disorder (Table 2). Several studies found that a significant decrease in platelet MAO activity was associated with the paranoid subtype[41]. Others found correlation between the presence of auditory hallucinations and reduced MAO activity[42]. Interestingly, a significant positive correlation was found between negative symptoms and platelet MAO activity in unmedicated male but not female schizophrenic patients[43]. These findings were only partially replicable by other studies. Attempts to relate disease prognosis with MAO activity have mostly failed, as enzyme activity was not related to either prognostic scores or age at onset of illness[44]. One study, however, showed that 36 patients with schizophrenia or schizoaffective disorders, who had had low platelet MAO activity, had significantly better social adjustment and fewer schizophrenic symptoms at several years follow-up[45]. Another study showed that patients with low platelet MAO activity are most likely to continue to manifest schizophrenic symptomatology in senium[46].

Table 2 Low monoamine oxidase activity in schizophrenia.
SubtypeParanoid schizophrenia
SymptomsAuditory hallucination
Negative symptoms in unmedicated males
PrognosisBetter social adjustment and fewer symptoms at follow-up (other study reported higher symptomatology in senium)

The controversial findings regarding platelet MAO activity in schizophrenia may, in part, stem from methodological differences as many factors can influence MAO activity. For example, mechanical stress to platelets, such as centrifugation speed, has been shown to significantly affect their MAO activity[47]. Conditions affecting the hematopoietic system can also affect platelet MAO activity, as it was shown to be enhanced in megaloblastic anemia and reduced in iron deficiency anemia and was significantly correlated with mean platelet volume, platelet protein densities and protein content per platelet[48]. MAO substrate concentration can also affect MAO activity, since it appears that some chronic schizophrenics have lower Michaelis-Menten constant (Km) or maximal velocity (Vmax). Interestingly, it was reported that as many as 75 percent of the studies used suboptimal substrate concentrations, which could have led to the low estimate of the MAO activity and erroneous comparisons between schizophrenia and normal groups[49].

Despite numerous reports of decreased platelet MAO activity in schizophrenia, this finding has not been generally accepted[50]. Twenty years of research trying to correlate MAO activity with genetics, medication, symptoms and prognosis led to inconsistent and sometimes nonreplicable results and therefore it could not be established as a biomarker for schizophrenia.

Serotonin

Human platelets possess organelles, whose function is closely related to that of 5-HT neurons, including an active transport system for 5-HT, binding sites for many drugs and neurotransmitters, dense bodies, which store 5-HT, and mitochondria expressing MAO-B[17]. Investigators have used different techniques to examine platelet 5-HT in psychiatry. Among the most common measurements are platelet 5-HT concentration, platelet 5-HT receptor density and 5-HT uptake. Naturally, the first studies on the serotonergic system were in patients with affective disorder, but the therapeutic success of serotonergic/dopaminergic antipsychotic drugs has directed attention towards the role of 5-HT in schizophrenia. One study found that platelet serotonin concentrations were significantly elevated in patients with chronic schizophrenia and in patients with bipolar depression[51]. Other studies showed that mean platelet 5-HT concentrations in patients with schizophrenia, mostly in paranoid schizophrenia and patients with psychotic depression, were higher than those of controls[52,53]. Hyperserotonemia was positively correlated with severity of auditory hallucinations and negatively correlated with lack of insight and conceptual disorganization. Hyposerotonemia, although less common than hyperserotonemia, was present in nonparanoid schizophrenia and in nonpsychotic depression; in the same study they also found an increase in platelet 5-HT concentration in schizophrenia, especially in a subgroup with a chronic active course of the disease compared to normal controls[53]. The gender differences in platelet 5-HT, with a significantly higher concentration in males than in females, were preserved in healthy and schizophrenia subjects[54] (Table 3).

Table 3 Platelet serotonin in schizophrenia.
High platelet serotoninLow platelet serotonin
Paranoid schizophreniaNon-paranoid schizophrenia
Correlated with severity of auditory hallucinationsCorrelated with lack of insight and with conceptual disorganization
Chronic active course
Higher levels in spring in patients with positive symptoms
Winter born patients

Contradictory findings were reported regarding the effect of antipsychotic drugs on platelet 5-HT. One study noted that antipsychotic drugs elevate blood 5-HT[55], while others have reported that antipsychotic medication seemed to decrease platelet 5-HT[56]. Other groups could not detect an association between any clinical feature or treatment and hyperserotonemia, except for significantly higher 5-HT levels in a subgroup of patients receiving benzodiazepines[57].

Seasonal variations in platelet 5-HT concentrations were observed in schizophrenic patients, with higher levels in spring in these patients compared with depressed patients and normal controls. Specifically, those changes were observed in patients with positive symptoms and clearly separated them from patients with negative schizophrenia. Birth season had no effect on 5HT levels in healthy subjects, while in patients with schizophrenia, birth-season had a significant effect, with highest levels observed in patients born in winter[58]. Daylight duration was probably not involved in the latter phenomenon, as a recent study was unable to detect an association between platelet 5-HT concentrations and duration of exposure of schizophrenic patients to daylight[59].

A complex relationship exists between the serotonergic system and the hypothalamic-pituitary-adrenal (HPA) axis[60]. One study observed that schizophrenia patients with dysregulated HPA axis activity, manifested by a high rate of non-suppression of the axis following dexamethasone administration (dexamethasone suppression test-DST), also show hypercortisolemia and platelet hyperserotonemia independent of the duration of the disease. These results were repeated by several additional studies[61]. In a recent study, however, a significant correlation between platelet 5-HT and plasma cortisol or plasma prolactin concentrations was observed in healthy controls, but not in schizophrenia or depressed patients[62].

Studies on 5HT receptor binding revealed that, in unmedicated schizophrenic patients, the maximal number (Bmax) of 5-HT2A receptors was significantly higher than that of control subjects. Typical antipsychotic medication significantly decreased Bmax values to a comparable level of the healthy controls[63]. In contrast, another showed that treatment with the atypical, new generation antipsychotic drug risperidone, which blocks 5-HT2A receptors with a higher efficiency, caused a significant increase in the 5-HT2A receptors[64]. Other groups found no significant difference between schizophrenic patients and normal subjects in the KD of the 5HT receptors and showed that treatment with typical antipsychotic drugs, such as haloperidol or thiothixine, did not affect its Bmax or KD[65]. These findings are in line with differences found in the Bmax of 5-HT2 binding sites that disappeared after correction for age and sex. An exception was schizophrenic patients who made suicide attempts and showed significantly higher Bmax than non-suicidal schizophrenic patients who did not differ from normal controls[66].

Unlike major depression, in which numerous studies have proposed imipramine binding to 5-HT transporter as a biological marker for the disorder[67,68], only a few studies were performed in schizophrenic patients. Some of them point at a tendency towards lower platelet 5-HT uptake in schizophrenia compared with healthy subjects[69-71], while others found no change due to the disease[71,72]. A more recent study found that the mean Bmax of serotonin transporter sites for schizophrenic patients without antipsychotic therapy was significantly higher than in normal controls. The Bmax values for schizophrenic patients on various antipsychotic drugs were significantly lower than those obtained from unmedicated schizophrenic patients and were comparable to those found in normal control subjects. The KD values in all subject groups remained unchanged[73].

Additional neurotransmitters

The study of other neurotransmitter systems in platelets in schizophrenia is scarce, even although a plethora of evidence from pharmacological, molecular, genetic and imaging studies point to abnormalities in dopamine and glutamate transmission in this disorder. One study compared dopamine uptake in platelets before and after treatment with typical antipsychotic drugs. No difference was observed between drug-free patients and healthy subjects. However, following treatment with typical antipsychotic drugs, the Km for platelet dopamine uptake increased by 76% in subjects with schizophrenia and decreased by 94% in subjects with non-schizophrenia psychoses[74]. Despite these confusing findings, attempts were made to identify a circulating inhibitor of platelet dopamine uptake in plasma of schizophrenic patients, which failed[75]. Glutamate receptor was also assessed using the intracellular calcium response to glutamate as a marker for its sensitivity. The response of the schizophrenic and depressed psychotic subjects to glutamate stimulation was significantly greater than control subjects (P < 0.005)[76]. Therefore, in 2009 Baier et al[77] suggested a fast flow-cytometric method to investigate glutamate receptor sensitivity in whole blood platelets as a future diagnostic tool. Although the link between schizophrenia and alterations in the adrenergic system is not well documented, adrenergic receptors in platelets were studied as well. For example, Rosen et al[78] reported that schizophrenic patients with relatively subsensitive platelet α-2-adrenergic receptors, as measured by clonidine binding, tend to have more negative symptoms and a diminished clinical response to chlorpromazine.

Alongside the gastrointestinal tract, platelets are the major source of serotonin in the periphery. Therefore, it is conceivable to expect that some of the impairments in the serotonin system in the CNS in schizophrenia will also be manifested in the periphery, as has been observed for other mental disorders. Despite the prominent role of dopamine and glutamate in the pathophysiology of schizophrenia, the findings in platelets regarding both neurotransmitters are less convincing, probably since platelets are not a major anatomical substrate for these transmitters.

AGGREGATION, PROSTAGLANDINS AND ARACHIDONIC ACID

In schizophrenia, the issue of blood clotting and aggregation was brought to clinical attention by the increased incidence of mortality caused by cardiovascular disease and the increased risk of thrombotic complication in schizophrenic patients treated with antipsychotics. Unexpectedly, in vitro studies show that antipsychotic drugs reduced response of blood platelets to ADP, a stimulator of platelet aggregation[79,80]. It was suggested that the blockade of the 5-HT2A receptors by the antipsychotic drugs is involved in the decreased platelet aggregation[81]. In drug-free schizophrenic patients, aggregation of platelets stimulated by collagen or by serotonin was significantly lower[82,83] and was negatively correlated with psychosis ratings[84]. Yet aggregation by ADP showed increased response[82] in unmedicated schizophrenic patients. It was also shown that the reduced collagen induced platelet aggregation remained decreased, despite clinical recovery following antipsychotic treatment[83]. In contrast, there are reports on indistinguishable aggregation responses to ADP, adrenaline, 5-HT or collagen of platelets derived from unmedicated schizophrenics compared to healthy controls[85].

Prostaglandins play a major role in platelet aggregation. Both prostaglandin deficiency[86] and excess[87] hypotheses have been proposed for the etiology of schizophrenia. In platelets, it was shown that ADP stimulates the synthesis of prostaglandin E1 (PGE1) in normal subjects and patients with affective illness but not in schizophrenic patients[88]. An interesting finding was revealed by studies on the inhibitory effects of PGE1 on the platelet aggregation response to ADP in schizophrenia[89-92]. The schizophrenic patients demonstrated hyposensitivity to PGE1, which was manifested by reduced PGE1 - stimulated cAMP accumulation compared to normal controls, suggesting a hyposensitivity of the platelet PGE receptor in schizophrenia. Kanof et al[92] showed that platelet cAMP response to PGE1 was negatively correlated with global symptom severity and with several indexes of positive symptom severity, but not with negative symptom severity. Focusing on the clinical characteristics of the subgroup showing platelet PGE1 subsensitivity revealed relatively successful heterosexual relationships, less anergia and more severe activation factor, as analyzed by the Brief Psychiatric Rating Scale (BPRS). Notably, MRI studies in schizophrenia demonstrated negative correlation between platelet PGE1 subsensitivity and the corpus callosum size[89]. Abnormalities in corpus callosum size and shape were observed in schizophrenia and suggested to be consistent with a hypothesis of decreased connectivity between the left and right hemispheres in the disorder[93].

Prostaglandin abnormalities in schizophrenia may stem from impairments in the metabolism of arachidonic acid, which is the precursor of prostaglandins and is essential for neuronal membrane activity. Platelets were found to emit a burst of chemiluminescence during incubation with arachidonic or linoleic acid (an essential fatty acid, which can be used in the biosynthesis of arachidonic acid). This chemiluminescence response indicates activation of prostaglandin synthase. One study demonstrated that platelets from drug naive schizophrenic subjects showed significantly increased arachidonic acid metabolism compared to control subjects. No significant difference was observed between schizophrenic and control subjects in their chemiluminescence response to linoleic acid. In schizophrenic subjects, treatment with antipsychotic drugs normalized the arachidonic acid overactive response, while linoleic acid response was unaffected[94]. Concomitantly, additional study showed that the activity of platelet phospholipase A2 (PLA2), which releases arachidonic acid from membrane phospholipids, was significantly increased in schizophrenia compared to healthy subjects and to patients with other psychiatric disorders. Antipsychotic treatment significantly reduced PLA2 activity[95].

The impairment in arachidonic acid metabolism can also stem from changes in the cytoplasmic membrane function and in the regulation of secondary messengers, for example, the phosphoinositide signaling system. This system is coupled to several neurotransmitter receptors in the central nervous system, stimulation of which induces phospholipase C (PLC) to catalyze the hydrolysis of plasma membrane phosphatidylinositol bisphosphate (PIP2) to inositol trisphosphate (IP3) and diacylglycerol[96]. The latter can be converted to arachidonic acid by PLC. No differences in the content or in precursor incorporation into PIP2 were found in platelets of drug-free schizophrenic patients[97]. Yao et al[18] proposed increased activity of PLC in schizophrenia, which resulted in increased production of the second messengers DAG and IP3, in response to thrombin, in normal control subjects as well as in schizophrenic patients before and after haloperidol withdrawal. Increase in IP3 and its binding to its receptors on the endoplasmic reticulum causes cytosolic concentration of calcium to increase. Indeed, Rípová et al[98] found increased cytosolic Ca2+ level in platelets in drug-free schizophrenic patients. Taken together, these data pinpoint arachidonic acid, which is a precursor for prostaglandins on one hand and an important component of cell membranes and major intracellular signaling pathways on the other hand, as a part of a continuum linking between neurotransmission dependent vulnerabilities and basic physiological changes, such as coagulation function. The recent interest in polyunsaturated fatty acids in schizophrenia may open new research directions in platelets in this disease.

OTHER NEW POTENTIAL BIOMARKER
Mitochondrial complex I

Mitochondria are the energy source that drives the biochemical processes involved in various cell functions, including neuronal activity. Neuronal activity can monitor synaptic connectivity associated with adaptive changes in emotional and cognitive function, all abnormal in schizophrenia. Several studies by our lab and by others showed mitochondrial dysfunction in schizophrenia. In platelets, an increase in the activity of mitochondrial complex I (COI) in medicated and unmedicated schizophrenic patients compared with control subjects and patients with affective disorders was observed[99]. In patients, COI increased activity was positively correlated with psychotic symptomology, while its decrease was observed in patients with residual schizophrenia[100]. Upon combining imaging studies of brain glucose uptake in rest with platelet COI activity, a disease-state dependent correlation between glucose metabolism (rCGM) in schizophrenia-relevant brain areas and platelets COI was observed. No such correlations were observed in healthy subjects, suggesting the correlation between peripheral COI activity and rCGM as a pathological factor that is differentially expressed in subgroups of schizophrenic patients[101]. Interestingly, abnormality in the expression of several subunits of COI and one of their transcription factors Sp1 was observed in platelets as well as in postmortem brain specimens of patients with schizophrenia[102].

Platelet auto-antibodies

As part of the autoimmune theory of schizophrenia, one group showed that platelet auto-antibodies (PAA) titers were significantly higher in schizophrenia patients than those in the control group[103]. Additional study showed positive correlation between social withdrawal retardation scores and the level of PAA and a negative correlation between PAA levels and depressive symptoms in chronic schizophrenic patients[104]. These results provide evidence for the existence of distinct correlations between peripheral autoantibodies, total psychometric scores and discrete symptom clusters of schizophrenia (BPRS subscales).

Glutamine synthetase-like protein

Glutamine synthetase-like protein (GSLP) is a glutamate-metabolizing enzyme in human platelets. It was reported that GSLP in platelets was significantly higher in chronic patients with schizophrenia than controls. Interestingly, similar increases in GSLP levels were also observed in schizophrenic patients’ prefrontal cortex, a brain area highly implicated in this disorder. Moreover, survival analysis of the group of patients chronically treated with the atypical antipsychotic drug olanzapine showed that the higher the level of platelets GSLP was before treatment, the shorter the treatment duration was required to achieve a positive clinical response[105].

Genomics based research in platelets of schizophrenic patients

Only few studies implicate the recent genomic advances in schizophrenia in platelet research. A recent study showed that the Src-family kinase Fyn, which is involved in brain development and emotional regulation, was lower in platelets of schizophrenic patients and their first degree relatives[106]. An additional study focusing on the disordered platelet phospholipid metabolism of schizophrenic patients found an association between PLA2 activity in platelets and the BanI of PLA2 reported in schizophrenic patients[107]. None of the proteins of more replicable genes in schizophrenia, such as DISC1 and neuregulin, were mentioned in platelet research.

A scheme summarizing the main methods used for platelets research in psychiatry and the findings with the most prevalent tendency of change identified in platelets of schizophrenic patients addressed in the present review is presented in Table 4 and in Figure 1.

Figure 1
Figure 1 Schematic presentation of the main impairments observed in platelets of patients with schizophrenia. Glu: Glutamate; GSLP: Glutamine synthase-like protein; 5HT: Serotonin; MAO: Monoamine oxidase; NT: neurotransmitters; PPA: Platelet auto-antibodies.
Table 4 Main methods used in psychiatry platelet research.
MethodBrief description and representative references
MAO activity measuring2-carbon14-tryptamine hydrochloride or carbon14-benzylamine as substrates[16,109]
Platelet 5-HT levelsHigh-performance liquid chromatography (HPLC) with fluorimetric or electrochemical detection[110]
Serotonin transporter binding sites[3H] Imipramine binding assay[73]
Noradrenergic binding sitesSpecific binding of 3H-clonidine and 3H-yohimbine to platelet membranes[111]
Prostaglandin E1 suppression of platelet aggregation responseUsing PGE1 to suppress platelet aggregation in response to ADP[89]
Platelet Associated Antibodies 11Enzyme linked immunoassay based on color development after reaction with anti-human immunoglobulin bound to horseradish peroxidase[112]
Platelet mitochondrial enzyme activityMeasuring different complexes of the respiratory system activity[100]
CONCLUSION

The endeavor of platelet research in schizophrenia is a thrilling lesson about tendencies in the history of science. The psychopharmacological revolution raised hope to find a definitive answer for the long running debate about the biological origin for mental disorders. Platelet research that started in the 1950s was one of the tools for establishing the bioaminergic theory of depression, which inspired the dopaminergic theory for schizophrenia. The finding of low MAO activity in schizophrenic patients’ platelets was a promising innovative tool that converges, in itself, scientific theory and clinical practice; reduced activity of an enzyme which is known to degrade dopamine in the central nervous system, which results in an increment of dopamine, in line with the dopaminergic theory prediction. MAO activity in platelets was expected to become a simple way for improving diagnostic methods, while the genetic and familial research was aimed at contributing to the development of prevention strategies. Hundreds of studies were published through the 70s and 80s but the inconsistency of the results led to a significant fading in the number of publications on platelets in general and on platelet MAO activity in particular.

The question is whether there is justification for the relative desertion of platelet research, a field of research which probably will not give us the ultimate answer but still may shed some light on the biological abnormalities in mental disorders. It seems that the same psychological processes in the attachment of scientists to their theory fuel their disappointment from unfulfilled pretentious wishes. In psychiatry, the combination of the critics interpreting a biochemical model to a psychiatric disorder which seems extremely reductionist and the subjective diagnostic criteria with many overlaps that manifests in a high percentage of comorbidity, amplifies the motivation to find a biomarker. This can, as Wirz-Justice claimed, lead to “too many ‘instant’ unreplicable studies”[108]. With the accumulating biological knowledge that entangled the old dopaminergic theory in schizophrenia, our wish to find a single marker for a single diagnosis seems further away than ever. However, totally disregarding the reported findings in platelets in schizophrenia bears the risk of throwing the baby out with the bathwater. An alternative is to make use of the following models: (1) One diagnosis - few biomarkers: equivalent and complementary to the diagnostic operational criteria for mental disorders (DSM-IV, ICD-10), one has to identify as many biomarkers as can be found in blood samples to support the clinical diagnosis; (2) Split diagnosis according to a single biomarker: define a set of clinical characteristics as a sub-diagnosis of schizophrenia that could be recognized by a single biomarker. For example, define a subgroup of schizophrenic patient with platelet PGE1 sub-sensitivity or altered mitochondrial functions; and (3) Biomarkers for symptoms: instead of characterizing the study groups using the current diagnosis criteria, patients’ groups will be defined according to symptoms. For example, studies in the past that correlated the presence of auditory hallucinations and reduced MAO activity or psychotic symptoms and hyperserotonemia. Such markers will help to overcome the complexity of validating subjective symptoms that are currently used for diagnosis or, as many clinician claim, as targets for treatment.

In conclusion, the ample data of parallel findings in brain and in platelets of abnormalities in various biological factors, such as mitochondrial related functions and GSLP, substantiate the relevance of platelets as proxies or a “peephole” to the inaccessible CNS and encourage further studies in platelets using modern, more sophisticated techniques. The latter should be based on analyses of an array of diverse biological factors that will serve to portray a profile of abnormalities, which will become a biomarker of this complex disorder.

Footnotes

Peer reviewer: Diego A Forero, Dr., Professor, School of Medicine, Universidad Antonio Nariño, Laboratories of Molecular Genetics, Bogotá, Colombia

S- Editor Lu YJ L- Editor Roemmele A E- Editor Zheng XM

References
1.  McGlashan TH. A selective review of recent North American long-term followup studies of schizophrenia. Schizophr Bull. 1988;14:515-542.  [PubMed]  [DOI]  [Cited in This Article: ]
2.  McGlashan TH, Carpenter WT. Long-term followup studies of schizophrenia: editors' introduction. Schizophr Bull. 1988;14:497-500.  [PubMed]  [DOI]  [Cited in This Article: ]
3.  Green MF. What are the functional consequences of neurocognitive deficits in schizophrenia. Am J Psychiatry. 1996;153:321-330.  [PubMed]  [DOI]  [Cited in This Article: ]
4.  McGlashan TH, Fenton WS. The positive-negative distinction in schizophrenia. Review of natural history validators. Arch Gen Psychiatry. 1992;49:63-72.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 177]  [Cited by in F6Publishing: 162]  [Article Influence: 5.1]  [Reference Citation Analysis (0)]
5.  Docherty NM, DeRosa M, Andreasen NC. Communication disturbances in schizophrenia and mania. Arch Gen Psychiatry. 1996;53:358-364.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 125]  [Cited by in F6Publishing: 109]  [Article Influence: 3.9]  [Reference Citation Analysis (0)]
6.  Dingman CW, McGlashan TH. Discriminating characteristics of suicides. Chestnut Lodge follow-up sample including patients with affective disorder, schizophrenia and schizoaffective disorder. Acta Psychiatr Scand. 1986;74:91-97.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 41]  [Cited by in F6Publishing: 41]  [Article Influence: 1.1]  [Reference Citation Analysis (0)]
7.  Tsuang MT. Suicide in schizophrenics, manics, depressives, and surgical controls. A comparison with general population suicide mortality. Arch Gen Psychiatry. 1978;35:153-155.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 164]  [Cited by in F6Publishing: 177]  [Article Influence: 3.8]  [Reference Citation Analysis (0)]
8.  McGlashan TH, Carpenter WT. Long-term followup studies of schizophrenia: editors' introduction. Schizophr Bull. 1988;14:497-500.  [PubMed]  [DOI]  [Cited in This Article: ]
9.  Pearse AG. The diffuse neuroendocrine system and the apud concept: related "endocrine" peptides in brain, intestine, pituitary, placenta, and anuran cutaneous glands. Med Biol. 1977;55:115-125.  [PubMed]  [DOI]  [Cited in This Article: ]
10.  Pletscher A, Da Prada M, Tranzer JP. Metabolism, subcellular transport and storage of 5-hydroxytryptamine in blood platelets. Ann Med Exp Biol Fenn. 1968;46:399-406.  [PubMed]  [DOI]  [Cited in This Article: ]
11.  Paasonen MK, Pletscher A. Increase of free 5-hydroxytryptamine in blood plasma by reserpine and a benzoquinolizine derivative. Experientia. 1959;15:477-479.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 29]  [Cited by in F6Publishing: 28]  [Article Influence: 1.1]  [Reference Citation Analysis (0)]
12.  Rapport MM, Green AA, Page IH. Serum vasoconstrictor, serotonin; isolation and characterization. J Biol Chem. 1948;176:1243-1251.  [PubMed]  [DOI]  [Cited in This Article: ]
13.  Gaddum JH. Antagonism between lysergic acid diethylamide and 5-hydroxytryptamine. J Physiol. 1953;121:15P.  [PubMed]  [DOI]  [Cited in This Article: ]
14.  Feldstein A, Hoagland H, Freeman H. Blood and urinary serotonin and 5-hydroxyindole acetic acid levels in schizophrenic patients and normal subjects. J Nerv Ment Dis. 1959;129:62-68.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 37]  [Cited by in F6Publishing: 35]  [Article Influence: 0.5]  [Reference Citation Analysis (0)]
15.  Todrick A, Tait AC, Marshall EF. Blood platelet 5-hydroxytryptamine levels in psychiatric patients. J Ment Sci. 1960;106:884-890.  [PubMed]  [DOI]  [Cited in This Article: ]
16.  Murphy DL, Wyatt RJ. Reduced monoamine oxidase activity in blood platelets from schizophrenic patients. Nature. 1972;238:225-226.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 265]  [Cited by in F6Publishing: 266]  [Article Influence: 5.1]  [Reference Citation Analysis (0)]
17.  Da Prada M, Cesura AM, Launay JM, Richards JG. Platelets as a model for neurones. Experientia. 1988;44:115-126.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 178]  [Cited by in F6Publishing: 164]  [Article Influence: 4.6]  [Reference Citation Analysis (0)]
18.  Yao JK, van Kammen DP. Incorporation of 3H-arachidonic acid into platelet phospholipids of patients with schizophrenia. Prostaglandins Leukot Essent Fatty Acids. 1996;55:21-26.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 11]  [Cited by in F6Publishing: 11]  [Article Influence: 0.4]  [Reference Citation Analysis (0)]
19.  Strunecká A, Rípová D. What can the investigation of phosphoinositide signaling system in platelets of schizophrenic patients tell us. Prostaglandins Leukot Essent Fatty Acids. 1999;61:1-5.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 12]  [Cited by in F6Publishing: 12]  [Article Influence: 0.5]  [Reference Citation Analysis (0)]
20.  Edmondson DE, Mattevi A, Binda C, Li M, Hubálek F. Structure and mechanism of monoamine oxidase. Curr Med Chem. 2004;11:1983-1993.  [PubMed]  [DOI]  [Cited in This Article: ]
21.  Youdim MB. Platelet monoamine oxidase B: use and misuse. Experientia. 1988;44:137-141.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 32]  [Cited by in F6Publishing: 34]  [Article Influence: 0.9]  [Reference Citation Analysis (0)]
22.  Sandler M, Reveley MA, Glover V. Human platelet monoamine oxidase activity in health and disease: a review. J Clin Pathol. 1981;34:292-302.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 75]  [Cited by in F6Publishing: 76]  [Article Influence: 1.8]  [Reference Citation Analysis (0)]
23.  Sparks DL, Woeltz VM, Markesbery WR. Alterations in brain monoamine oxidase activity in aging, Alzheimer's disease, and Pick's disease. Arch Neurol. 1991;48:718-721.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 61]  [Cited by in F6Publishing: 63]  [Article Influence: 1.9]  [Reference Citation Analysis (0)]
24.  Belmaker RH, Murphy DL, Wyatt RJ, Loriaux DL. Human platelet monoamine oxidase changes during the menstrual cycle. Arch Gen Psychiatry. 1974;31:553-556.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 96]  [Cited by in F6Publishing: 96]  [Article Influence: 1.9]  [Reference Citation Analysis (0)]
25.  Wahlund LO, Sääf J, Eneroth-Grimfors E, Nilsson BA, Bevegård S. Reduced platelet MAO activity during pregnancy. Acta Physiol Scand. 1986;126:135-138.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 7]  [Cited by in F6Publishing: 7]  [Article Influence: 0.2]  [Reference Citation Analysis (0)]
26.  Schalling D, Asberg M, Edman G, Oreland L. Markers for vulnerability to psychopathology: temperament traits associated with platelet MAO activity. Acta Psychiatr Scand. 1987;76:172-182.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 326]  [Cited by in F6Publishing: 322]  [Article Influence: 8.7]  [Reference Citation Analysis (0)]
27.  Engström G, Alling C, Gustavsson P, Oreland L, Träskman-Bendz L. Clinical characteristics and biological parameters in temperamental clusters of suicide attempters. J Affect Disord. 1997;44:45-55.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 33]  [Cited by in F6Publishing: 31]  [Article Influence: 1.1]  [Reference Citation Analysis (0)]
28.  Yatham LN, Srisurapanont M, Zis AP, Kusumakar V. Comparative studies of the biological distinction between unipolar and bipolar depressions. Life Sci. 1997;61:1445-1455.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 23]  [Cited by in F6Publishing: 23]  [Article Influence: 0.9]  [Reference Citation Analysis (0)]
29.  Cameron OG, Nesse RM. Systemic hormonal and physiological abnormalities in anxiety disorders. Psychoneuroendocrinology. 1988;13:287-307.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 59]  [Cited by in F6Publishing: 58]  [Article Influence: 1.6]  [Reference Citation Analysis (0)]
30.  Gottfries CG. Alzheimer's disease and senile dementia: biochemical characteristics and aspects of treatment. Psychopharmacology (Berl). 1985;86:245-252.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 132]  [Cited by in F6Publishing: 133]  [Article Influence: 3.4]  [Reference Citation Analysis (0)]
31.  von Knorring AL, Hallman J, von Knorring L, Oreland L. Platelet monoamine oxidase activity in type 1 and type 2 alcoholism. Alcohol Alcohol. 1991;26:409-416.  [PubMed]  [DOI]  [Cited in This Article: ]
32.  Pedersen NL, Oreland L, Reynolds C, McClearn GE. Importance of genetic effects for monoamine oxidase activity in thrombocytes in twins reared apart and twins reared together. Psychiatry Res. 1993;46:239-251.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 57]  [Cited by in F6Publishing: 57]  [Article Influence: 1.8]  [Reference Citation Analysis (0)]
33.  Baron M, Levitt M, Gruen R, Kane J, Asnis L. Platelet monoamine oxidase activity and genetic vulnerability to schizophrenia. Am J Psychiatry. 1984;141:836-842.  [PubMed]  [DOI]  [Cited in This Article: ]
34.  Baron M, Risch N, Levitt M, Gruen R. Genetic analysis of platelet monoamine oxidase activity in families of schizophrenic patients. J Psychiatr Res. 1985;19:9-21.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 17]  [Cited by in F6Publishing: 17]  [Article Influence: 0.4]  [Reference Citation Analysis (0)]
35.  Baron M, Levitt M. Platelet monoamine oxidase activity: relation to genetic load of schizophrenia. Psychiatry Res. 1980;3:69-74.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 19]  [Cited by in F6Publishing: 18]  [Article Influence: 0.4]  [Reference Citation Analysis (0)]
36.  Duncavage M, Luchins DJ, Meltzer HY. Platelet MAO activity and family history of schizophrenia. Psychiatry Res. 1982;7:47-51.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 11]  [Cited by in F6Publishing: 11]  [Article Influence: 0.3]  [Reference Citation Analysis (0)]
37.  Campbell M, Friedman E, Green WH, Small AM, Burdock EI. Blood platelet monoamine oxidase activity in schizophrenic children and their families. A preliminary study. Neuropsychobiology. 1976;2:239-246.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 16]  [Cited by in F6Publishing: 17]  [Article Influence: 0.4]  [Reference Citation Analysis (0)]
38.  Marcolin MA, Davis JM. Platelet monoamine oxidase in schizophrenia: a meta-analysis. Schizophr Res. 1992;7:249-267.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 23]  [Cited by in F6Publishing: 24]  [Article Influence: 0.8]  [Reference Citation Analysis (0)]
39.  Kemali D, Maj M, Ariano MG, Salvati A. Platelet monoamine-oxidase activity in schizophrenia. Relation to genetic load of the illness and treatment with antipsychotic drugs. Encephale. 1985;11:31-34.  [PubMed]  [DOI]  [Cited in This Article: ]
40.  Ho BT, Smith RC, Kralik P, Allen R, Schoolar J, Khan M, DeJohn C. Effects of neuroleptics on platelet monoamine oxidase activity. Biol Psychiatry. 1982;17:885-895.  [PubMed]  [DOI]  [Cited in This Article: ]
41.  Meltzer HY, Zureick JL. Relationship of auditory hallucinations and paranoia to platelet MAO activity in schizophrenics: sex and race interactions. Psychiatry Res. 1987;22:99-109.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 14]  [Cited by in F6Publishing: 13]  [Article Influence: 0.4]  [Reference Citation Analysis (0)]
42.  Schildkraut JJ, Orsulak PJ, Schatzberg AF, Herzog JM. Platelet monoamine oxidase activity in subgroups of schizophrenic disorders. Schizophr Bull. 1980;6:220-225.  [PubMed]  [DOI]  [Cited in This Article: ]
43.  Lewine RJ, Meltzer HY. Negative symptoms and platelet monoamine oxidase activity in male schizophrenic patients. Psychiatry Res. 1984;12:99-109.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 26]  [Cited by in F6Publishing: 24]  [Article Influence: 0.6]  [Reference Citation Analysis (0)]
44.  Mann J, Thomas KM. Platelet monoamine oxidase activity in schizophrenia. Relationship to disease, treatment, institutionalization and outcome. Br J Psychiatry. 1979;134:366-371.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 27]  [Cited by in F6Publishing: 27]  [Article Influence: 0.6]  [Reference Citation Analysis (0)]
45.  Malas KL, van Kammen DP. Platelet MAO activity and long-term outcome in schizophrenia and schizoaffective disorder. Am J Psychiatry. 1983;140:794-796.  [PubMed]  [DOI]  [Cited in This Article: ]
46.  Bridge TP, Jeste DV, Wise CD, Potkin SG, Phelps BH, Wyatt RJ. Schizophrenic outcome in late life: symptom state and platelet monoamine oxidase activity. Psychiatry Res. 1984;11:91-97.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 3]  [Cited by in F6Publishing: 2]  [Article Influence: 0.1]  [Reference Citation Analysis (0)]
47.  Jackman HL, Meltzer HY. Factors affecting determination of platelet monoamine oxidase activity. Schizophr Bull. 1980;6:259-266.  [PubMed]  [DOI]  [Cited in This Article: ]
48.  Sullivan JL, Coffey CE, Sullivan PD, Taska R, Mahorney S, Cavenar JO. Metabolic factors affecting monoamine oxidase activity. Schizophr Bull. 1980;6:308-313.  [PubMed]  [DOI]  [Cited in This Article: ]
49.  Wise CD, Potkin SG, Bridge TP, Phelps BH, Cannon-Spoor H, Wyatt RJ. Sources of error in the determination of platelet monoamine oxidase: a review of methods. Schizophr Bull. 1980;6:245-253.  [PubMed]  [DOI]  [Cited in This Article: ]
50.  Fleissner A, Seifert R, Schneider K, Eckert W, Fuisting B. Platelet monoamine oxidase activity and schizophrenia--a myth that refuses to die. Eur Arch Psychiatry Neurol Sci. 1987;237:8-15.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 8]  [Cited by in F6Publishing: 8]  [Article Influence: 0.2]  [Reference Citation Analysis (0)]
51.  Stahl SM, Woo DJ, Mefford IN, Berger PA, Ciaranello RD. Hyperserotonemia and platelet serotonin uptake and release in schizophrenia and affective disorders. Am J Psychiatry. 1983;140:26-30.  [PubMed]  [DOI]  [Cited in This Article: ]
52.  Jackman H, Luchins D, Meltzer HY. Platelet serotonin levels in schizophrenia: relationship to race and psychopathology. Biol Psychiatry. 1983;18:887-902.  [PubMed]  [DOI]  [Cited in This Article: ]
53.  Mück-Seler D, Jakovljević M, Deanović Z. Platelet serotonin in subtypes of schizophrenia and unipolar depression. Psychiatry Res. 1991;38:105-113.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 39]  [Cited by in F6Publishing: 37]  [Article Influence: 1.1]  [Reference Citation Analysis (0)]
54.  Mück-Seler D, Pivac N, Jakovljević M. Sex differences, season of birth and platelet 5-HT levels in schizophrenic patients. J Neural Transm. 1999;106:337-347.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 24]  [Cited by in F6Publishing: 24]  [Article Influence: 1.0]  [Reference Citation Analysis (0)]
55.  Joseph MH, Owen F, Baker HF, Bourne RC. Platelet serotonin concentration and monoamine oxidase activity in unmedicated chronic schizophrenic and in schizoaffective patients. Psychol Med. 1977;7:159-162.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 34]  [Cited by in F6Publishing: 32]  [Article Influence: 0.7]  [Reference Citation Analysis (0)]
56.  Garelis E, Gillin JC, Wyatt RJ, Neff N. Elevated blood serotonin concentrations in unmediated chronic schizophrenic patients: a preliminary study. Am J Psychiatry. 1975;132:184-186.  [PubMed]  [DOI]  [Cited in This Article: ]
57.  Kolakowska T, Molyneux SG. Platelet serotonin concentration in schizophrenic patients. Am J Psychiatry. 1987;144:232-234.  [PubMed]  [DOI]  [Cited in This Article: ]
58.  Jakovljević M, Mück-Seler D, Pivac N, Ljubicić D, Bujas M, Dodig G. Seasonal influence on platelet 5-HT levels in patients with recurrent major depression and schizophrenia. Biol Psychiatry. 1997;41:1028-1034.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 34]  [Cited by in F6Publishing: 32]  [Article Influence: 1.2]  [Reference Citation Analysis (0)]
59.  Ljubicić D, Stipcević T, Pivac N, Jakovljević M, Mück-Seler D. The influence of daylight exposure on platelet 5-HT levels in patients with major depression and schizophrenia. J Photochem Photobiol B. 2007;89:63-69.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 13]  [Cited by in F6Publishing: 13]  [Article Influence: 0.8]  [Reference Citation Analysis (0)]
60.  Dinan TG. Serotonin and the regulation of hypothalamic-pituitary-adrenal axis function. Life Sci. 1996;58:1683-1694.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 225]  [Cited by in F6Publishing: 232]  [Article Influence: 8.3]  [Reference Citation Analysis (0)]
61.  Mück-Seler D, Pivac N, Jakovljević M, Brzović Z. Platelet serotonin, plasma cortisol, and dexamethasone suppression test in schizophrenic patients. Biol Psychiatry. 1999;45:1433-1439.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 61]  [Cited by in F6Publishing: 62]  [Article Influence: 2.5]  [Reference Citation Analysis (0)]
62.  Muck-Seler D, Pivac N, Mustapic M, Crncevic Z, Jakovljevic M, Sagud M. Platelet serotonin and plasma prolactin and cortisol in healthy, depressed and schizophrenic women. Psychiatry Res. 2004;127:217-226.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 72]  [Cited by in F6Publishing: 72]  [Article Influence: 3.6]  [Reference Citation Analysis (0)]
63.  Govitrapong P, Chagkutip J, Turakitwanakan W, Srikiatkhachorn A. Platelet 5-HT(2A) receptors in schizophrenic patients with and without neuroleptic treatment. Psychiatry Res. 2000;96:41-50.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 16]  [Cited by in F6Publishing: 15]  [Article Influence: 0.6]  [Reference Citation Analysis (0)]
64.  Arranz B, Rosel P, Sarró S, Ramirez N, Dueñas R, Cano R, María Sanchez J, San L. Altered platelet serotonin 5-HT2A receptor density but not second messenger inositol trisphosphate levels in drug-free schizophrenic patients. Psychiatry Res. 2003;118:165-174.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 21]  [Cited by in F6Publishing: 20]  [Article Influence: 1.0]  [Reference Citation Analysis (0)]
65.  Pandey SC, Sharma RP, Janicak PG, Marks RC, Davis JM, Pandey GN. Platelet serotonin-2 receptors in schizophrenia: effects of illness and neuroleptic treatment. Psychiatry Res. 1993;48:57-68.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 19]  [Cited by in F6Publishing: 20]  [Article Influence: 0.6]  [Reference Citation Analysis (0)]
66.  Arora RC, Meltzer HY. Serotonin2 receptor binding in blood platelets of schizophrenic patients. Psychiatry Res. 1993;47:111-119.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 29]  [Cited by in F6Publishing: 27]  [Article Influence: 0.9]  [Reference Citation Analysis (0)]
67.  Langer SZ, Sette M, Raisman R, Briley M. High-affinity binding of 3H-imipramine in brain and platelets: relevance to depression and antidepressant drugs. Adv Biochem Psychopharmacol. 1982;31:171-177.  [PubMed]  [DOI]  [Cited in This Article: ]
68.  Langer SZ, Zarifian E, Briley M, Raisman R, Sechter D. High-affinity 3H-imipramine binding: a new biological marker in depression. Pharmacopsychiatria. 1982;15:4-10.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 43]  [Cited by in F6Publishing: 43]  [Article Influence: 1.0]  [Reference Citation Analysis (0)]
69.  Rotman A, Shatz A, Szekely GA. Correlation between serotonin uptake and imipramine binding in schizophrenic patients. Prog Neuropsychopharmacol Biol Psychiatry. 1982;6:57-61.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 20]  [Cited by in F6Publishing: 20]  [Article Influence: 0.5]  [Reference Citation Analysis (0)]
70.  Rotman A, Zemishlany Z, Munitz H, Wijsenbeek H. The active uptake of serotonin by platelets of schizophrenic patients and their families: possibility of a genetic marker. Psychopharmacology (Berl). 1982;77:171-174.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 17]  [Cited by in F6Publishing: 17]  [Article Influence: 0.4]  [Reference Citation Analysis (0)]
71.  Wood PL, Suranyi-Cadotte BE, Nair NP, LaFaille F, Schwartz G. Lack of association between [3H]imipramine binding sites and uptake of serotonin in control, depressed and schizophrenic patients. Neuropharmacology. 1983;22:1211-1214.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 54]  [Cited by in F6Publishing: 54]  [Article Influence: 1.3]  [Reference Citation Analysis (0)]
72.  Weizman A, Gonen N, Tyano S, Szekely GA, Rehavi M. Platelet [3H]imipramine binding in autism and schizophrenia. Psychopharmacology (Berl). 1987;91:101-103.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 21]  [Cited by in F6Publishing: 21]  [Article Influence: 0.6]  [Reference Citation Analysis (0)]
73.  Govitrapong P, Mukda S, Turakitwanakan W, Dumrongphol H, Chindaduangratn C, Sanvarinda Y. Platelet serotonin transporter in schizophrenic patients with and without neuroleptic treatment. Neurochem Int. 2002;41:209-216.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 12]  [Cited by in F6Publishing: 13]  [Article Influence: 0.6]  [Reference Citation Analysis (0)]
74.  Dean B, Sundram S, Hill C, Copolov DL. Platelet [3H]dopamine uptake is differentially affected by neuroleptic drug treatment in schizophrenia and schizophreniform disorder. Prog Neuropsychopharmacol Biol Psychiatry. 1996;20:45-55.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 4]  [Cited by in F6Publishing: 4]  [Article Influence: 0.1]  [Reference Citation Analysis (0)]
75.  Sundram S, Dean B, Copolov DL. The development of a method to measure [3H] dopamine uptake by washed platelets provides no evidence for circulating inhibitors of platelet dopamine uptake in schizophrenia. Biol Psychiatry. 1994;36:595-600.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 6]  [Cited by in F6Publishing: 4]  [Article Influence: 0.1]  [Reference Citation Analysis (0)]
76.  Berk M, Plein H, Belsham B. The specificity of platelet glutamate receptor supersensitivity in psychotic disorders. Life Sci. 2000;66:2427-2432.  [PubMed]  [DOI]  [Cited in This Article: ]
77.  Baier PC, Koch JM, Seeck-Hirschner M, Ohlmeyer K, Wilms S, Aldenhoff JB, Hinze-Selch D. A flow-cytometric method to investigate glutamate-receptor-sensitivity in whole blood platelets - results from healthy controls and patients with schizophrenia. J Psychiatr Res. 2009;43:585-591.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 16]  [Cited by in F6Publishing: 16]  [Article Influence: 1.1]  [Reference Citation Analysis (0)]
78.  Rosen J, Silk KR, Rice HE, Smith CB. Platelet alpha-2-adrenergic dysfunction in negative symptom schizophrenia: a preliminary study. Biol Psychiatry. 1985;20:539-545.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 10]  [Cited by in F6Publishing: 10]  [Article Influence: 0.3]  [Reference Citation Analysis (0)]
79.  Dietrich-Muszalska A, Rabe-Jablonska J, Nowak P, Kontek B. The first- and second-generation antipsychotic drugs affect ADP-induced platelet aggregation. World J Biol Psychiatry. 2010;11:268-275.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 21]  [Cited by in F6Publishing: 21]  [Article Influence: 1.5]  [Reference Citation Analysis (0)]
80.  Dietrich-Muszalska A, Rabe-Jabłońska J, Olas B. The effects of the second generation antipsychotics and a typical neuroleptic on collagen-induced platelet aggregation in vitro. World J Biol Psychiatry. 2010;11:293-299.  [PubMed]  [DOI]  [Cited in This Article: ]
81.  Blasco-Fontecilla H, Baca-Garcia E, de Leon J. Do atypical antipsychotic drugs reduce the risk of ischemic heart disease and mortality Possible role of 5-HT2A receptor blockade. Schizophr Res. 2010;119:160-163.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 11]  [Cited by in F6Publishing: 11]  [Article Influence: 0.8]  [Reference Citation Analysis (0)]
82.  Dietrich-Muszalska A, Olas B. Modifications of blood platelet proteins of patients with schizophrenia. Platelets. 2009;20:90-96.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 24]  [Cited by in F6Publishing: 23]  [Article Influence: 1.5]  [Reference Citation Analysis (0)]
83.  McAdams C, Leonard BE. Changes in platelet aggregatory responses to collagen and 5-hydroxytryptamine in depressed, schizophrenic and manic patients. Int Clin Psychopharmacol. 1992;7:81-85.  [PubMed]  [DOI]  [Cited in This Article: ]
84.  Yao JK, van Kammen DP, Gurklis J, Peters JL. Platelet aggregation and dense granule secretion in schizophrenia. Psychiatry Res. 1994;54:13-24.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 24]  [Cited by in F6Publishing: 23]  [Article Influence: 0.8]  [Reference Citation Analysis (0)]
85.  Dinan TG. Neuroleptic effects on platelet aggregation: a study in normal volunteers and schizophrenics. Psychol Med. 1987;17:875-881.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 11]  [Cited by in F6Publishing: 11]  [Article Influence: 0.3]  [Reference Citation Analysis (0)]
86.  Horrobin DF. Schizophrenia as a prostaglandin deficiency disease. Lancet. 1977;1:936-937.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 140]  [Cited by in F6Publishing: 143]  [Article Influence: 3.0]  [Reference Citation Analysis (0)]
87.  Feldberg W. Possible association of schizophrenia with a disturbance in prostaglandin metabolism: a physiological hypothesis. Psychol Med. 1976;6:359-369.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 44]  [Cited by in F6Publishing: 44]  [Article Influence: 0.9]  [Reference Citation Analysis (0)]
88.  Abdulla YH, Hamadah K. Effect of ADP on PGE1 formation in blood platelets from patients with depression, mania and schizophrenia. Br J Psychiatry. 1975;127:591-595.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 75]  [Cited by in F6Publishing: 75]  [Article Influence: 1.5]  [Reference Citation Analysis (0)]
89.  Kaiya H. Prostaglandin E1 suppression of platelet aggregation response in schizophrenia. Schizophr Res. 1991;5:67-80.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 12]  [Cited by in F6Publishing: 12]  [Article Influence: 0.4]  [Reference Citation Analysis (0)]
90.  Kaiya H, Ofuji M, Nozaki M, Tsurumi K. Platelet prostaglandin E1 hyposensitivity in schizophrenia: decrease in cyclic AMP formation and in inhibitory effects on aggregation. Psychopharmacol Bull. 1990;26:381-384.  [PubMed]  [DOI]  [Cited in This Article: ]
91.  Ofuji M, Kaiya H, Nozaki M, Tsurumi K. Platelet prostaglandin E1 hyposensitivity in schizophrenia: reduction of prostaglandin E1- or forskolin-stimulated cyclic AMP response in platelets. Life Sci. 1989;45:2135-2140.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 6]  [Cited by in F6Publishing: 6]  [Article Influence: 0.2]  [Reference Citation Analysis (0)]
92.  Kanof PD, Davidson M, Johns CA, Mohs RC, Davis KL. Clinical correlates of platelet prostaglandin receptor subsensitivity in schizophrenia. Am J Psychiatry. 1987;144:1556-1560.  [PubMed]  [DOI]  [Cited in This Article: ]
93.  Downhill JE, Buchsbaum MS, Wei T, Spiegel-Cohen J, Hazlett EA, Haznedar MM, Silverman J, Siever LJ. Shape and size of the corpus callosum in schizophrenia and schizotypal personality disorder. Schizophr Res. 2000;42:193-208.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 128]  [Cited by in F6Publishing: 132]  [Article Influence: 5.5]  [Reference Citation Analysis (0)]
94.  Das I, Khan NS. Increased arachidonic acid induced platelet chemiluminescence indicates cyclooxygenase overactivity in schizophrenic subjects. Prostaglandins Leukot Essent Fatty Acids. 1998;58:165-168.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 47]  [Cited by in F6Publishing: 45]  [Article Influence: 1.7]  [Reference Citation Analysis (0)]
95.  Gattaz WF, Schmitt A, Maras A. Increased platelet phospholipase A2 activity in schizophrenia. Schizophr Res. 1995;16:1-6.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 125]  [Cited by in F6Publishing: 121]  [Article Influence: 4.2]  [Reference Citation Analysis (0)]
96.  Odagaki Y, Koyama T. Epinephrine- and thrombin-stimulated high-affinity GTPase activity in platelet membranes from patients with psychiatric disorders. Psychiatry Res. 2002;112:111-119.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 10]  [Cited by in F6Publishing: 10]  [Article Influence: 0.5]  [Reference Citation Analysis (0)]
97.  Essali MA, Das I, de Belleroche J, Hirsch SR. The platelet polyphosphoinositide system in schizophrenia: the effects of neuroleptic treatment. Biol Psychiatry. 1990;28:475-487.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 35]  [Cited by in F6Publishing: 35]  [Article Influence: 1.0]  [Reference Citation Analysis (0)]
98.  Rípová D, Strunecká A, Nemcová V, Farská I. Phospholipids and calcium alterations in platelets of schizophrenic patients. Physiol Res. 1997;46:59-68.  [PubMed]  [DOI]  [Cited in This Article: ]
99.  Ben-Shachar D, Zuk R, Gazawi H, Reshef A, Sheinkman A, Klein E. Increased mitochondrial complex I activity in platelets of schizophrenic patients. Int J Neuropsychopharmacol. 1999;2:245-253.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 83]  [Cited by in F6Publishing: 85]  [Article Influence: 3.4]  [Reference Citation Analysis (0)]
100.  Dror N, Klein E, Karry R, Sheinkman A, Kirsh Z, Mazor M, Tzukerman M, Ben-Shachar D. State-dependent alterations in mitochondrial complex I activity in platelets: a potential peripheral marker for schizophrenia. Mol Psychiatry. 2002;7:995-1001.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 110]  [Cited by in F6Publishing: 110]  [Article Influence: 5.2]  [Reference Citation Analysis (0)]
101.  Ben-Shachar D, Bonne O, Chisin R, Klein E, Lester H, Aharon-Peretz J, Yona I, Freedman N. Cerebral glucose utilization and platelet mitochondrial complex I activity in schizophrenia: A FDG-PET study. Prog Neuropsychopharmacol Biol Psychiatry. 2007;31:807-813.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 56]  [Cited by in F6Publishing: 56]  [Article Influence: 3.3]  [Reference Citation Analysis (0)]
102.  Ben-Shachar D, Karry R. Sp1 expression is disrupted in schizophrenia; a possible mechanism for the abnormal expression of mitochondrial complex I genes, NDUFV1 and NDUFV2. PLoS One. 2007;2:e817.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 68]  [Cited by in F6Publishing: 63]  [Article Influence: 3.7]  [Reference Citation Analysis (0)]
103.  Spivak B, Schechtman M, Blumensohn R, Schonherz-Pine Y, Yoran-Hegesh R, Deckmann M, Mayer R, Weizman A, Shinitzky M. Blind verification of elevated platelet autoantibodies in serum of schizophrenic patients--part I: young subjects. Neuropsychobiology. 2009;60:44-48.  [PubMed]  [DOI]  [Cited in This Article: ]
104.  Sinyakov MS, Kessler B, Karp L, Weizman A, Kessler AR. Platelet-associated antibodies mediate protective autoimmune response in schizophrenia. Neuropsychobiology. 2003;48:41-46.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 7]  [Cited by in F6Publishing: 7]  [Article Influence: 0.3]  [Reference Citation Analysis (0)]
105.  Burbaeva GSh, Boksha IS, Tereshkina EB, Savushkina OK, Turishcheva MS, Starodubtseva LI, Brusov OS, Morozova MA. Effect of olanzapine treatment on platelet glutamine synthetase-like protein and glutamate dehydrogenase immunoreactivity in schizophrenia. World J Biol Psychiatry. 2006;7:75-81.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 15]  [Cited by in F6Publishing: 15]  [Article Influence: 0.8]  [Reference Citation Analysis (0)]
106.  Hattori K, Fukuzako H, Hashiguchi T, Hamada S, Murata Y, Isosaka T, Yuasa S, Yagi T. Decreased expression of Fyn protein and disbalanced alternative splicing patterns in platelets from patients with schizophrenia. Psychiatry Res. 2009;168:119-128.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 17]  [Cited by in F6Publishing: 19]  [Article Influence: 1.3]  [Reference Citation Analysis (0)]
107.  Barbosa NR, Junqueira RM, Vallada HP, Gattaz WF. Association between BanI genotype and increased phospholipase A2 activity in schizophrenia. Eur Arch Psychiatry Clin Neurosci. 2007;257:340-343.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 28]  [Cited by in F6Publishing: 26]  [Article Influence: 1.5]  [Reference Citation Analysis (0)]
108.  Wirz-Justice A. Platelet research in psychiatry. Experientia. 1988;44:145-152.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 50]  [Cited by in F6Publishing: 50]  [Article Influence: 1.4]  [Reference Citation Analysis (0)]
109.  White HL, McLeod MN, Davidson JR. Platelet monoamine oxidase activity in schizophrenia. Am J Psychiatry. 1976;133:1191-1193.  [PubMed]  [DOI]  [Cited in This Article: ]
110.  Nebinger P, Koel M. Automatic determination of serotonin in biological fluids by liquid chromatography. J Chromatogr. 1988;427:326-330.  [PubMed]  [DOI]  [Cited in This Article: ]
111.  García-Sevilla JA, Hollingsworth PJ, Smnith CB. Alpha 2-adrenoreceptors on human platelets: selective labelling by [3H]clonidine and [3H]yohimbine and competitive inhibition by antidepressant drugs. Eur J Pharmacol. 1981;74:329-341.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 97]  [Cited by in F6Publishing: 98]  [Article Influence: 2.3]  [Reference Citation Analysis (0)]
112.  Shinitzky M, Deckmann M, Kessler A, Sirota P, Rabbs A, Elizur A. Platelet autoantibodies in dementia and schizophrenia. Possible implication for mental disorders. Ann N Y Acad Sci. 1991;621:205-217.  [PubMed]  [DOI]  [Cited in This Article: ]  [Cited by in Crossref: 22]  [Cited by in F6Publishing: 22]  [Article Influence: 0.7]  [Reference Citation Analysis (0)]