Pharmacological properties of the mouse neurotensin receptor 3. Maintenance of cell surface receptor during internalization of neurotensin

FEBS Lett. 2001 Apr 20;495(1-2):100-5. doi: 10.1016/s0014-5793(01)02367-5.

Abstract

We recently reported the molecular identification of a new type of receptor for the neuropeptide neurotensin (NT), the neurotensin receptor 3 (NTR3), identical to sortilin, which binds receptor-associated protein. Here, we demonstrate that the cloned mouse NTR3 is expressed on the plasma membrane of transfected COS-7 cells. The mouse NTR3 is detectable by photoaffinity labeling and immunoblotting at the cell surface as a 100 kDa N-glycosylated protein. Biochemical analysis and confocal microscopic imaging clearly indicate that NT is efficiently internalized after binding to NTR3, and that despite this internalization, the amount of receptor present on the cell surface is maintained.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Vesicular Transport
  • Animals
  • Biological Transport
  • Blotting, Western
  • COS Cells
  • Cell Membrane / metabolism
  • Gene Expression
  • Immunoblotting
  • Iodine Radioisotopes
  • Membrane Glycoproteins / genetics
  • Membrane Glycoproteins / metabolism*
  • Mice
  • Microscopy, Confocal
  • Molecular Weight
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / metabolism*
  • Neurotensin / metabolism*
  • Neurotensin / pharmacokinetics
  • Photoaffinity Labels
  • Radioligand Assay
  • Receptors, Neurotensin / genetics
  • Receptors, Neurotensin / metabolism
  • Subcellular Fractions / metabolism
  • Substrate Specificity
  • Transfection

Substances

  • Adaptor Proteins, Vesicular Transport
  • Iodine Radioisotopes
  • Membrane Glycoproteins
  • Nerve Tissue Proteins
  • Photoaffinity Labels
  • Receptors, Neurotensin
  • Neurotensin
  • sortilin