Target-selected mutagenesis of the rat

Bart M G Smits; Josine Mudde; Ronald H A Plasterk; Edwin Cuppen

doi:10.1016/j.ygeno.2003.08.010

Target-selected mutagenesis of the rat

Genomics. 2004 Feb;83(2):332-4. doi: 10.1016/j.ygeno.2003.08.010.

Authors

Bart M G Smits¹, Josine Mudde, Ronald H A Plasterk, Edwin Cuppen

Affiliation

¹ Functional Genomics Group, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands.

PMID: 14706462
DOI: 10.1016/j.ygeno.2003.08.010

Abstract

The rat is one of the most extensively studied model organisms, and with its genome being sequenced, tools to manipulate gene function in vivo have become increasingly important. We here report proof of principle for target-selected mutagenesis as a reverse genetic or knockout approach for the rat.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
DNA Mutational Analysis
Ethylnitrosourea
Fertility / genetics
Follicle Stimulating Hormone / genetics
Germ-Line Mutation
Mutagenesis* / drug effects
Mutagens
Rats
Rats, Mutant Strains
Rats, Wistar

Substances

Mutagens
Follicle Stimulating Hormone
Ethylnitrosourea