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Neuronal sources of theta rhythm in the entorhinal cortex of the rat

I. Laminar distribution of theta field potentials

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Summary

The laminar distribution of theta (θ) field potentials in the entorhinal cortex (EC) was investigated in paralysed and locally anesthetized rats injected with physostigmine in order to induce θ rhythm. Electrode penetrations through the medial, intermediate and lateral subdivisions of the EC showed in all cases: 1. the presence of θ rhythm from layer VI to layer III approximately in phase with CA1 θ rhythm; 2. an amplitude minimum between the outer third of layer III and the inner half of layer I; and 3. a phase-reversed θ rhythm in layers II-I with an amplitude maximum in the outer half of layer I. Results indicate the existence of neuronal sources of θ rhythm in the EC.

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References

  • Adey WR, Dunlow CW, Hendrix CE (1960) Hippocampal slow waves: distribution and phase relations in the course of approach learning. Arch Neurol 3: 74–90

    Google Scholar 

  • Alonso A, García-Austt E (1987) Neuronal sources of theta rhythm in the entorhinal cortex of the rat. II. Phase relations between unit discharges and theta field potentials. Exp Brain Res 67: 502–509

    Google Scholar 

  • Alonso A, Gaztelu JM, Buño W Jr, García-Austt E (1987) Crosscorrelation analysis of septohippocampal neurons during θ rhythm. Brain Res (in press)

  • Alonso A, Köhler C (1984) A study of the reciprocal connections between the septum and the entorhinal area using anterograde and retrograde axonal transport methods in the rat brain. J Comp Neurol 275: 327–343

    Google Scholar 

  • Andersen P, Holmqvist B, Voorhoeve PE (1966a) Entorhinal activation of dentate granule cells. Acta Physiol Scand 66: 448–460

    Google Scholar 

  • Andersen P, Holmqvist B, Voorhoeve PE (1966b) Excitatory synapses on hippocampal apical dendrites activated by entorhinal stimulation. Acta Physiol Scand 66: 461–472

    Google Scholar 

  • Apostol G, Creutzfeldt OD (1974) Crosscorrelation between the activity of septal units and hippocampal EEG during arousal. Brain Res 67: 65–75

    Google Scholar 

  • Baisden RH, Woodruff ML, Hoover DB (1984) Cholinergic and non-cholinergic septo-hippocampal projections. A doublelabel horseradish peroxidase-acetylcholinesterase study in the rabbit. Brain Res 290: 146–151

    Google Scholar 

  • Bendat JS, Piersol AG (1971) Random data: analysis and measurement procedures. Wiley & Sons, New York

    Google Scholar 

  • Blackstad TW (1956) Commissural connections of the hippocampal region in the rat, with special reference to their mode of termination. J Comp Neurol 105: 417–437

    Google Scholar 

  • Bland BH, Andersen P, Ganes T (1975) Two generators of hippocampal theta activity in rabbits. Brain Res 94: 199–218

    Google Scholar 

  • Bland SK, Bland BH (1986) Medial septal modulation of hippocampal theta cell discharges. Brain Res 375: 102–116

    Google Scholar 

  • Bland BH, Whishaw IQ (1976) Generators and topography of hippocampal theta (RSA) in the anaesthetized and freely moving rat. Brain Res 118: 259–280

    Google Scholar 

  • Boeijinga PH, Van Groen Th (1984) Inputs from the olfactory bulb to the entorhinal cortex in the cat. II. Physiological studies. Exp Brain Res 57: 40–48

    Google Scholar 

  • Buño W Jr, Fuentes J, Segundo JP (1978) Crayfish stretchreceptor organs: effects of length-steps with and without perturbations. Biol Cybern 31: 99–110

    Google Scholar 

  • Buzsáki G, Leung L-WS, Vanderwolf CH (1983) Cellular base of hippocampal EEG in the behaving rat. Brain Res Rev 6: 139–171

    Google Scholar 

  • Buzsáki G, Czopf J, Kondákor I, Kellény L (1986) Laminar distribution of hippocampal rhythmic slow activity (RSA) in the behaving rat: current-source density analysis, effects of urethane and atropine. Brain Res 365: 125–137

    Google Scholar 

  • Cox DR, Lewis PAW (1966) The statistical analysis of series of events. Wiley & Sons, New York

    Google Scholar 

  • Faingold CL, Hoffmann WE, Caspary DM (1984) Effects of iontophoretic application of convulsants on the sensory responses of neurons in the brain-stem reticular formation. Electroencephalogr Clin Neurophysiol 58: 55–64

    Google Scholar 

  • Feenstra BWA, Holsheimer J (1979) Dipole-like neuronal sources of theta rhythm in dorsal hippocampus, dentate gyrus and cingulate cortex of the urethane-anaesthetized rat. Electroencephalogr Clin Neurophysiol 47: 532–538

    Google Scholar 

  • Gaztelu JM, Buño W Jr (1982) Septo-hippocampal relationships during EEG theta rhythm. Electroencephalogr Clin Neurophysiol 54: 375–387

    Google Scholar 

  • Gaztelu JM, Dajas F, Sanchez-Arroyos R, García-Austt E (1981) [14C]2-deoxyglucosa mapping of active hippocampal areas during theta rhythm induced by curarimimetics in the rat. Neurosci Lett Suppl 7: 46

    Google Scholar 

  • Gogolak G, Stumpf CH, Petsche H, Stere S (1968) The firing pattern of septal neurons and the form of the hippocampal theta wave. Brain Res 7: 201–207

    Google Scholar 

  • Green JD, Arduini AA (1954) Hippocampal electrical activity in arousal. J Neurophysiol 17: 403–420

    Google Scholar 

  • Green KF, Rawlins JNP (1979) Hippocampal theta in rats under urethane: generators and phase relations. Electroenchephalogr Clin Neurophysiol 47: 420–429

    Google Scholar 

  • Hellon RI (1971) The marking of electrode tip positions in nervous tissue. J Physiol (Lond) 214: 12P

  • Herreras O, Solis JM, Lerma J (1986) Abolition of CA1 population spike by sensory stimulation. Exp Brain Res 61: 654–657

    Google Scholar 

  • Holmes JE, Adey WR (1960) Electrical activity of the entorhinal cortex during conditioned behaviour. Am J Physiol 199: 741–744

    Google Scholar 

  • Holsheimer J, Stok CJ, Lopes da Silva FH (1983) Theta rhythm related hippocampal cell discharges in the urethane anaesthetized rat: evidence for a predominant entorhinal input. Electroencephalogr Clin Neurophysiol 55: 464–467

    Google Scholar 

  • Köhler C, Chan-Palay V, Wu JY (1984) Septal neurons containing glutamic acid decarboxylase immunoreactivity project to the hippocampal region in the rat brain. Anat Embryol 169: 41–44

    Google Scholar 

  • Krettek JE, Price JL (1977) Projections from the amygdaloid complex and adjacent olfactory structures to the entorhinal cortex and to the subiculum in the rat and cat. J Comp Neurol 177: 723–752

    Google Scholar 

  • Leung L-WS (1984) Model of gradual phase shift of theta rhythm in the rat. J Neurophysiol 52: 1051–1065

    Google Scholar 

  • Lorente de Nó R (1933) Studies on the structure of the cerebral cortex. I. The area entorhinalis. J Psychol Neurol (Lpz) 5: 381–438

    Google Scholar 

  • Lorente de Nó R (1934) Studies on the structure of the cerebral cortex. II. Continuation of the study of the ammonic system. J Psychol Neurol (Lpz) 46: 113–177

    Google Scholar 

  • Macadar O, Roig JA, Monti JM, Budelli R (1970) The functional relationship between septal and hippocampal unit activity and hippocampal theta rhythm. Physiol Behav 5: 1443–1449

    Google Scholar 

  • Mitchell SJ, Ranck JB Jr (1980) Generation of theta rhythm in medial entorhinal cortex of freely moving rats. Brain Res 178: 49–66

    Google Scholar 

  • Mitchell SJ, Rawlins JNP, Steward O, Olton DS (1982) Medial septal area lesions disrupt θ rhythm and cholinergic staining in medial entorhinal cortex and produce impaired radial arm maze behavior in rats. J Neurosci 2: 292–302

    Google Scholar 

  • Petsche H, Stumpf CH, Gogolak G (1962) The significance of the rabbit septum as a relay station between the midbrain and hippocampus. I. The control of hippocampal arousal activity by the septum cells. Electroencephalogr Clin Neurophysiol 14: 202–211

    Google Scholar 

  • Ramön y Cajal S (1911) Histologie du système nerveux l'homme et des vertébrés. Maloine, Paris

    Google Scholar 

  • Rawlins JNP, Feldon J, Gray JA (1979) Septo-hippocampal connections and the hippocampal theta rhythm. Exp Brain Res 37: 49–63

    Google Scholar 

  • Schwarz SP, Coleman PD (1981) Neurons of origin of the perforant path. Exp Neurol 74: 305–312

    Google Scholar 

  • Steward O (1976) Topographic organization of the projections from the entorhinal area to hippocampal formation of the rat. J Comp Neurol 167: 285–314

    Google Scholar 

  • Steward O, Scoville SA (1976) The cells of origin of entorhinal afferents to the hippocampus and fascia dentata of the rat. J Comp Neurol 169: 347–370

    Google Scholar 

  • Vanderwolf CH, Leung L-WS (1983) Hippocampal rhythmical slow activity: a brief history and the effects of entorhinal lesions and phencyclidine. In: Scifert W (ed) Neurobiology of the hippocampus. Academic Press, London, pp 275–302

    Google Scholar 

  • Winson J (1974) Patterns of hippocampal theta rhythm in the freely moving rat. Electroencephalogr Clin Neurophysiol 26: 291–301

    Google Scholar 

  • Winson J (1976a) Hippocampal theta rhythm. I. Depth profiles in the curarized rat. Brain Res 103: 57–70

    Google Scholar 

  • Winson J (1976b) Hippocampal theta rhythm. II. Depth profiles in the freely moving rat. Brain Res 103: 71–79

    Google Scholar 

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Author notes

  1. A. Alonso

    Present address: Department of Physiology and Biophysics, New York University Medical Center, 550 First Avenue, 10016, New York, NY, USA

Authors and Affiliations

  1. Departamento de Investigación, Hospital “Ramón y Cajal”, Ctra. Colmenar Km 9, E-28034, Madrid, Spain

    A. Alonso & E. García-Austt

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  1. A. Alonso
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  2. E. García-Austt
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Alonso, A., García-Austt, E. Neuronal sources of theta rhythm in the entorhinal cortex of the rat. Exp Brain Res 67, 493–501 (1987). https://doi.org/10.1007/BF00247282

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  • DOI: https://doi.org/10.1007/BF00247282

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